Human IL-2 ELISpot Kit
Cat# MBS335700-5x96-PC
Size : 5x96Wells(Pre-coated-plate)
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
Il-2 ELISpot Kit for Human
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS335700Principle of the assay: A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any… More details
Product Overview
Online Datasheet in PDFProduct Name
IL-2, ELISpot Kit
Full Product Name
Human IL-2 ELISpot Kit
Product Gene Name
IL-2 elispot kit
Product Synonym Names
IL-2
Species Reactivity
Human. No cross reactivity with other human cytokines. Cross reactivity with simian IL-2.
Specificity
The assay recognizes natural human IL-2. To define specificity of this IL-2 antibody pair, several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested (IL-1alpha, IL-1beta, IL-10 IL-12, IFNgamma, IL-4, IL-6, TNFalpha, IL-8 and IL-13). This testing was performed using the equivalent human IL-2 antibody pair in an ELISA assay.
Molecular Weight
4,762 Da
Intended Uses
ELISpot is a highly specific immunoassay for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. The ELISpot assay constitutes an ideal tool in the investigation of Th1 / Th2 responses, vaccine development, viral infection monitoring and treatment, cancerology, infectious disease, autoimmune diseases and tranplantation. Utilising sandwich immuno-enzyme technology, ELISpot assays can detect both secreted cytokines and single cells that simultaneously produce multiple cytokines. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
Product Categories/Family for IL-2 elispot kit
Human ELISpot Kit
Product References and Citations for IL-2 elispot kit
Dosset, M. et al., Clin Cancer Res., 2012; 18(22): 6284-97
Rinaldi, M. et al.,Thorax, 2012; 10.1136/thoraxjnl-2011-200690
Van Gulck, E. R. et al, J. Virol,2008; 82(7): 3561-3573.
Rinaldi, M. et al.,Thorax, 2012; 10.1136/thoraxjnl-2011-200690
Van Gulck, E. R. et al, J. Virol,2008; 82(7): 3561-3573.
Other Notes
Small volumes of IL-2 elispot kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for IL-2 purchase
MBS335700 is an ELISpot kit targeting IL-2. Also known as Human IL-2 ELISpot Kit. Reactive in Human. No cross reactivity with other human cytokines. Cross reactivity with simian IL-2..
Additional Details
NCBI/Uniprot data below describe general gene information for IL-2. It may not necessarily be applicable to this product.
NCBI Official Full Name
IL-2, partial
NCBI Official Synonym Full Names
interleukin 2
NCBI Official Symbol
IL2
NCBI Official Synonym Symbols
IL-2; TCGF; lymphokine
UniProt Protein Name
IL-2 protein
UniProt Synonym Protein Names
IL-2 protein
UniProt Gene Name
IL-2
UniProt Entry Name
O95974_HUMAN
NCBI Protein Information
interleukin-2
NCBI Summary for IL-2
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for IL-2
IL2: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. Belongs to the IL-2 family.
Protein type: Secreted, signal peptide; Cytokine; Oncoprotein; Secreted
Chromosomal Location of Human Ortholog: 4q26-q27
Cellular Component: extracellular space; extracellular region
Molecular Function: growth factor activity; interleukin-2 receptor binding; kinase activator activity; cytokine activity; carbohydrate binding; glycosphingolipid binding; kappa-type opioid receptor binding
Biological Process: positive regulation of isotype switching to IgG isotypes; negative regulation of heart contraction; natural killer cell activation; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; elevation of cytosolic calcium ion concentration; negative regulation of protein amino acid phosphorylation; cell-cell signaling; positive regulation of cell proliferation; protein kinase C activation; positive regulation of B cell proliferation; cell adhesion; T cell differentiation; positive regulation of interleukin-17 production; regulation of T cell homeostatic proliferation; positive regulation of regulatory T cell differentiation; positive regulation of immunoglobulin secretion; positive regulation of cell growth; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; positive regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; immune response; negative regulation of B cell apoptosis; negative regulation of apoptosis; positive regulation of inflammatory response
Show more Protein type: Secreted, signal peptide; Cytokine; Oncoprotein; Secreted
Chromosomal Location of Human Ortholog: 4q26-q27
Cellular Component: extracellular space; extracellular region
Molecular Function: growth factor activity; interleukin-2 receptor binding; kinase activator activity; cytokine activity; carbohydrate binding; glycosphingolipid binding; kappa-type opioid receptor binding
Biological Process: positive regulation of isotype switching to IgG isotypes; negative regulation of heart contraction; natural killer cell activation; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; elevation of cytosolic calcium ion concentration; negative regulation of protein amino acid phosphorylation; cell-cell signaling; positive regulation of cell proliferation; protein kinase C activation; positive regulation of B cell proliferation; cell adhesion; T cell differentiation; positive regulation of interleukin-17 production; regulation of T cell homeostatic proliferation; positive regulation of regulatory T cell differentiation; positive regulation of immunoglobulin secretion; positive regulation of cell growth; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; positive regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; immune response; negative regulation of B cell apoptosis; negative regulation of apoptosis; positive regulation of inflammatory response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with IL-2 elispot kit
| Products by Pathway | Pathway Diagram |
|---|---|
| ARMS-mediated Activation Pathway antibodies | ARMS-mediated Activation Pathway Diagram |
| AhR Pathway antibodies | AhR Pathway Diagram |
| Allograft Rejection Pathway antibodies | Allograft Rejection Pathway Diagram |
| Allograft Rejection Pathway antibodies | Allograft Rejection Pathway Diagram |
| Autoimmune Thyroid Disease Pathway antibodies | Autoimmune Thyroid Disease Pathway Diagram |
| Autoimmune Thyroid Disease Pathway antibodies | Autoimmune Thyroid Disease Pathway Diagram |
| Axon Guidance Pathway antibodies | Axon Guidance Pathway Diagram |
| Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies | Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram |
| Calcium Signaling In The CD4+ TCR Pathway antibodies | Calcium Signaling In The CD4+ TCR Pathway Diagram |
| Chagas Disease (American Trypanosomiasis) Pathway antibodies | Chagas Disease (American Trypanosomiasis) Pathway Diagram |