Glass slide multiplex ELISA array quantitatively detects 40 human immune response-related biomarkers. Suitable for all liquid sample types.
Human Immune Response Array Q1
Human Immune Response Array Q1
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 40 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| CD14
| CD163
| CD40 (TNFRSF5)
| CRP (C-Reactive Protein)
| E-Selectin
|
| Fas (TNFRSF6/Apo-1)
| Fas Ligand (TNFSF6)
| GCSF
| ICAM-1 (CD54)
| IL-1 alpha (IL-1 F1)
|
| IL-1 beta (IL-1 F2)
| IL-1 R4 (ST2)
| IL-10
| IL-12 p70
| IL-13
|
| IL-18
| IL-2
| IL-2 R alpha
| IL-4
| IL-6
|
| IL-8 (CXCL8)
| Lipocalin-2 (NGAL)
| MCP-1 (CCL2)
| MCP-2 (CCL8)
| MIF
|
| MIP-1 alpha (CCL3)
| MIP-1 beta (CCL4)
| Osteopontin (SPP1)
| PAI-1
| Platelet Factor 4 (CXCL4)
|
| Procalcitonin
| RAGE
| Resistin
| Thrombomodulin
| TNF alpha
|
| TREM-1
| Troponin I
| uPAR
| VCAM-1 (CD106)
| VEGF-A
|
Application Notes
Suggested Application
- Multiplexed Protein Detection
- Biomarker Screening
- Identifying Key Factors
- Confirming Biological Process
- Biomarker Validation
- Validation of Antibody Array Results
- Quantitative Protein Detection
- Establishing Normal Range
Kit Components
- Human Immune Response Array Q1 Slide
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAH-IMR-1.gal)
Excel (QAH-IMR-1_Analysis_Tool.xls)
citations
High serum urokinase-type plasminogen activator receptor correlates with sepsis-induced cardiomyopathy in sepsis patients
- Species:
- Human
- Sample Type:
- Serum (sepsis patients and healthy controls)
- Year:
- 2026
- Journal Name:
- Frontiers in Medicine
- PubMed ID:
- 42040567
Macrophage plasticity and differentiation on the decellularized human cornea
- Species:
- Human
- Sample Type:
- THP-1 cell
- Year:
- 2023
- Journal Name:
- Journal of Materials Research
Profile of soluble factors in pleural effusions predict prognosis in mesothelioma
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (pleural effusion supernatant)
- Year:
- 2021
- Journal Name:
- Cancer Biomarkers
- PubMed ID:
- 34487023
