RayBio® G-Series Human Inflammation Antibody Array 3 Kit. Detects 40 Human Inflammatory Factors. Suitable for all liquid sample types.
Human Inflammation Array G3
Human Inflammation Array G3
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Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 40 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| GCSF
| GM-CSF
| ICAM-1 (CD54)
|
IFN-gamma
| I-309 (TCA-3/CCL1)
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
|
IL-3
| IL-4
| IL-6
| IL-6 R
| IL-7
|
IL-8 (CXCL8)
| IL-10
| IL-11
| IL-12 p40
| IL-12 p70
|
IL-13
| IL-15
| IL-16
| IL-17A
| IP-10 (CXCL10)
|
MCP-1 (CCL2)
| MCP-2 (CCL8)
| M-CSF
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
|
MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
| RANTES (CCL5)
| TGF beta 1
| TNF alpha
|
TNF beta (TNFSF1B)
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
| PDGF-BB
| TIMP-2
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Inflammation Antibody Array G3 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-AAH-INF-G3-4.gal)
GAL (GAL-AAH-INF-G3-8.gal)
Excel (AAH-INF-G3_Analysis_Tool.xls)
citations
Serglycin secreted by late-stage nucleus pulposus cells is a biomarker of intervertebral disc degeneration
- Year:
- 2024
- Journal Name:
- Nature Communications
- PubMed ID:
- 38167807
Cadherin-11 cooperates with inflammatory factors to promote the migration and invasion of fibroblast-like synoviocytes in pigmented villonodular synovitis
- Species:
- Human
- Sample Type:
- Other Body Fluid (knee synovial fluid)
- Year:
- 2020
- Journal Name:
- Theranostics
- PubMed ID:
- 32929367
Human ESC-derived expandable hepatic organoids enable therapeutic liver repopulation and pathophysiological modeling of alcoholic liver injury
- Species:
- Human
- Sample Type:
- Conditioned Media (Organoid Liver model)
- Year:
- 2019
- Journal Name:
- Cell Research
ASIC1a induces synovial inflammation via the Ca 2+ /NFATc3/ RANTES pathway
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant
- Year:
- 2019
- Journal Name:
- Theranostics
- PubMed ID:
- 31903118
The alteration of uterine microbiota participated in the activation of the decidual inflammatory response in early spontaneous abortion
- Species:
- Human
- Sample Type:
- Other / Unspecified (decidual endometria)
- Year:
- 2025
- Journal Name:
- PLoS ONE
- PubMed ID:
- 39992946
Severe Hepatitis Promotes Hepatocellular Carcinoma Recurrence via NF-B Pathway-Mediated Epithelial-Mesenchymal Transition after Resection
- Species:
- Human
- Sample Type:
- Tissue lysate (Hepatic cancer tissue lysate with control)
- Year:
- 2016
- Journal Name:
- Clinical Cancer Research
- PubMed ID:
- 26655845
Angiogenic and innate immune responses triggered by PRP in tendon cells are not modified by hyperuricemia
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (tendon cell supernatants)
- Year:
- 2019
- Journal Name:
- Muscles Ligaments and Tendons Journal
- PubMed ID:
- 25489545
Granulocytosis and thrombocytosis in renal cell carcinoma: a pro-inflammatory cytokine response originating in the tumour
- Species:
- Human
- Sample Type:
- Tissue Lysate (Renal tumor lystae)
- Year:
- 2009
- Journal Name:
- Netharlands Journal of Medicine
- PubMed ID:
- 19581670
Proteome analysis of retinal glia cells-related inflammatory cytokines in the aqueous humour of diabetic patients
- Species:
- Human
- Sample Type:
- Aqueous Humor (Diabetic vs normal aqueos humor samples)
- Year:
- 2016
- Journal Name:
- Acta Ophtalmologica
- PubMed ID:
- 26268591
NaHCO3- and NaCl-Type Hot Springs Enhance the Secretion of Inflammatory Cytokine Induced by Polyinosinic-Polycytidylic Acid in HaCaT Cells
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (cell culture supernatants of HaCaT cells)
- Year:
- 2021
- Journal Name:
- Annals of Dermatology
- PubMed ID:
- 34616125
