Human Junction Plakoglobin (JUP) ELISA Kit
Cat# MBS2018947-96
Size : 96-Strip-Wells
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
Junction Plakoglobin (JUP) ELISA Kit for Human
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2018947SPECIAL PROMOTION: Get FREE Starbucks gift card ( for 48-Wells-Kit or for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment
This human JUP ELISA kit is a sandwich immunoassay for the quantitative measurement of junction plakoglobin in tissue homogenates and cell lysates, supporting desmosomal adhesion and arrhythmogenic cardiomyopathy research. Principle of the Assay: The… More details
Product Overview
Online Datasheet in PDFProduct Name
Junction Plakoglobin (JUP), ELISA Kit
Full Product Name
Human Junction Plakoglobin (JUP) ELISA Kit
Product Gene Name
JUP elisa kit
Product Synonym Names
DP3; gCat; G-Cat; CTNNG; DPIII; PDGB; PKGB; Gamma Catenin; Desmoplakin III; Desmoplakin-3
Species Reactivity
Human
Assay Type
Quantitative Sandwich
Samples
Human tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.056ng/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of JUP. No significant cross-reactivity or interference between JUP and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level JUP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level JUP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Molecular Weight
81,745 Da
OMIM
173325
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Categories/Family for JUP elisa kit
CD & Adhesion molecule
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Product References and Citations for JUP elisa kit
Ovarian Blood Sampling Identifies Junction Plakoglobin as a Novel Biomarker of Early Ovarian Cancer; Front Oncol; 2020 Sep 25;10:1767.; PMID: 33102207; Florian Weiland; Adelaide Proteomics Centre, The University of Adelaide, Adelaide, SA, Australia.
Other Notes
Small volumes of JUP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for JUP purchase
MBS2018947 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Junction Plakoglobin (JUP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing JUP. The ELISA analytical biochemical technique of the MBS2018947 kit is based on JUP antibody-JUP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect JUP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, JUP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for JUP. It may not necessarily be applicable to this product.
NCBI Official Full Name
junction plakoglobin
NCBI Official Synonym Full Names
junction plakoglobin
NCBI Official Symbol
JUP
NCBI Official Synonym Symbols
DP3; PDGB; PKGB; CTNNG; DPIII; ARVD12
UniProt Protein Name
Junction plakoglobin
UniProt Synonym Protein Names
Catenin gamma; Desmoplakin III; Desmoplakin-3
Protein Family
Junction plakoglobin
UniProt Gene Name
JUP
UniProt Synonym Gene Names
CTNNG; DP3
UniProt Entry Name
PLAK_HUMAN
NCBI Protein Information
junction plakoglobin
NCBI Summary for JUP
This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for JUP
CTNNG: Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. Homodimer. Component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1 and gamma- catenin/JUP, and possibly alpha-catenin/CTNNA1; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Interacts with MUC1. Interacts with CAV1. Interacts with PTPRJ. Interacts with DSC2. Belongs to the beta-catenin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: desmosome; internal side of plasma membrane; focal adhesion; apicolateral plasma membrane; intermediate filament; zonula adherens; catenin complex; intercellular junction; fascia adherens; Z disc; cytosol; actin cytoskeleton; cell-cell adherens junction; cytoskeleton; hemidesmosome; cytoplasm; plasma membrane; nucleus; lateral plasma membrane
Molecular Function: protein binding; signal transducer activity; protein homodimerization activity; structural constituent of cell wall; cadherin binding; transcription coactivator activity; structural molecule activity; protein kinase binding; protein phosphatase binding; nuclear hormone receptor binding; alpha-catenin binding
Biological Process: skin development; intercellular junction assembly and maintenance; cell-cell adhesion; cell migration; positive regulation of protein import into nucleus; cytoskeletal anchoring; protein heterooligomerization; regulation of cell fate specification; detection of mechanical stimulus; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; vascular endothelial growth factor receptor signaling pathway; regulation of cell proliferation
Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 12; Naxos Disease
Show more Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: desmosome; internal side of plasma membrane; focal adhesion; apicolateral plasma membrane; intermediate filament; zonula adherens; catenin complex; intercellular junction; fascia adherens; Z disc; cytosol; actin cytoskeleton; cell-cell adherens junction; cytoskeleton; hemidesmosome; cytoplasm; plasma membrane; nucleus; lateral plasma membrane
Molecular Function: protein binding; signal transducer activity; protein homodimerization activity; structural constituent of cell wall; cadherin binding; transcription coactivator activity; structural molecule activity; protein kinase binding; protein phosphatase binding; nuclear hormone receptor binding; alpha-catenin binding
Biological Process: skin development; intercellular junction assembly and maintenance; cell-cell adhesion; cell migration; positive regulation of protein import into nucleus; cytoskeletal anchoring; protein heterooligomerization; regulation of cell fate specification; detection of mechanical stimulus; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; vascular endothelial growth factor receptor signaling pathway; regulation of cell proliferation
Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 12; Naxos Disease
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with JUP elisa kit
| Reference Product | PubMed Publications |
|---|---|
| CTNNA1 elisa kit | >86 publications with JUP and CTNNA1 |
| DSP elisa kit | >39 publications with JUP and DSP |
| DSG2 elisa kit | >28 publications with JUP and DSG2 |
| CDH1 elisa kit | >5 publications with JUP and CDH1 |
| CDH2 elisa kit | >4 publications with JUP and CDH2 |
| SRC elisa kit | >3 publications with JUP and SRC |
| FYN elisa kit | >2 publications with JUP and FYN |
| EGFR elisa kit | >2 publications with JUP and EGFR |
Pathways associated with JUP elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Acute Myeloid Leukemia Pathway antibodies | Acute Myeloid Leukemia Pathway Diagram |
| Acute Myeloid Leukemia Pathway antibodies | Acute Myeloid Leukemia Pathway Diagram |
| Adherens Junctions Interactions Pathway antibodies | Adherens Junctions Interactions Pathway Diagram |
| Arf6 Trafficking Events Pathway antibodies | Arf6 Trafficking Events Pathway Diagram |
| Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies | Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram |
| Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies | Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram |
| Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies | Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram |
| Cell Junction Organization Pathway antibodies | Cell Junction Organization Pathway Diagram |
| Cell-Cell Communication Pathway antibodies | Cell-Cell Communication Pathway Diagram |
| Cell-cell Junction Organization Pathway antibodies | Cell-cell Junction Organization Pathway Diagram |
Diseases associated with JUP elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Heart Diseases Antibodies | >48 publications with JUP and Heart Diseases |
| Heart Defects, Congenital Antibodies | >38 publications with JUP and Heart Defects, Congenital |
| Adenocarcinoma Antibodies | >36 publications with JUP and Adenocarcinoma |
| Breast Neoplasms Antibodies | >21 publications with JUP and Breast Neoplasms |
| Lung Diseases Antibodies | >15 publications with JUP and Lung Diseases |
| Kidney Diseases Antibodies | >10 publications with JUP and Kidney Diseases |
| Cell Transformation, Neoplastic Antibodies | >9 publications with JUP and Cell Transformation, Neoplastic |
| Liver Neoplasms Antibodies | >8 publications with JUP and Liver Neoplasms |
| Liver Diseases Antibodies | >8 publications with JUP and Liver Diseases |
| Disease Models, Animal Antibodies | >7 publications with JUP and Disease Models, Animal |
Organs/Tissues associated with JUP elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Heart Antibodies | >44 publications with JUP and Heart |
| Skin Antibodies | >26 publications with JUP and Skin |
| Lung Antibodies | >24 publications with JUP and Lung |
| Muscle Antibodies | >20 publications with JUP and Muscle |
| Lymph Antibodies | >19 publications with JUP and Lymph |
| Lymph Node Antibodies | >19 publications with JUP and Lymph Node |
| Blood Antibodies | >18 publications with JUP and Blood |
| Kidney Antibodies | >12 publications with JUP and Kidney |
| Mouth Antibodies | >11 publications with JUP and Mouth |
| Liver Antibodies | >10 publications with JUP and Liver |
You might also be interested by the following products:
Cat#
Description
Cond.
Price Bef. VAT