Reagents and instruments for immunology, cell biology and molecular biology.
 
> Human/Mouse AKT Pathway Phosphorylation Array C1

Human/Mouse AKT Pathway Phosphorylation Array C1

Print Print
Please log in

Cat# AAH-AKT-1-8

Size : 8SampleKit

Brand : RayBiotech

Contact local distributor :


Phone : +1 850 650 7790

Human/Mouse AKT Pathway Phosphorylation Array C1RayBiotech's AKT Pathway Phosphorylation Antibody Array was used to evaluate OLFML3's impact on ErbB-related signaling. Figure adapted from Zhu et al., J Nanobiotechnol, 2023.RayBiotech's AKT Pathway Phosphorylation Antibody Array was used to assess the impact of SYK deletion on PI3K/AKT signaling in microglia from 5xFAD Alzheimer’s model mice. Figure adapted from Ennerfelt et al., Cell, 2022.RayBiotech's AKT Pathway Phosphorylation Antibody Array was used to assess the effects of NLRP6 knockdown on PI3K/AKT signaling in glioblastoma cells. Figure adapted from Zhi et al., Nat Commun, 2023.RayBiotech's AKT Pathway Phosphorylation Antibody Array was used to assess PI3K/AKT/mTOR activation in cervical epithelial lesions. Figure adapted from Li et al., Adv Sci, 2024.RayBiotech's AKT Pathway Phosphorylation Antibody Array was used to assess exosomal miR-9's effects on PDK/AKT signaling in endothelial cells. Figure adapted from Lu et al., J Exp Clin Cancer Res, 2018.RayBiotech's AKT Pathway Phosphorylation Antibody Array was used to evaluate EE02's impact on EGFR-driven signaling in MDA-MB-468 cells. Figure adapted from Li et al., J Exp Clin Cancer Res, 2019.Human/Mouse AKT Pathway Phosphorylation Array C1Human/Mouse AKT Pathway Phosphorylation Array C1

Human/Mouse AKT Pathway Phosphorylation Array C1

Human/Mouse AKT Pathway Phosphorylation Array C1

Detects 18 different phosphorylated human and mouse proteins crucial to the AKT Pathway
Contact Us for Bulk Request

Product Description

Specifications

Size2 Sample Kit, 4 Sample Kit, 8 Sample Kit
SpeciesHuman, Mouse
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeSemi-Quantitative
Number of Targets Detected18
Recommended ApplicationsMultiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Solid Support
Membrane
Design PrincipleSandwich-based
Method Of DetectionChemiluminescence
Research AreaPost-Translational Modifications, Phosphorylation, AMPK Signaling, HER/ErbB Signaling, HIF-1 alpha Signaling, IGF Signaling, JAK/STAT Signaling, MAPK Signaling, mTOR Signaling, PI3K-AKT Signaling, TGF-beta Signaling, Wnt-beta-Catenin Signaling, Akt Signaling
Shipping TypeBlue ice
Storage-20°C

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Akt (P-Ser473)
(PKB (P-Ser473))
AMPKa (P-Thr172)
  • Gene Symbol: PRKAA1 AMPK1
  • Gene ID: 5562
  • Accession #: Q13131
BAD (P-Ser112)
4E-BP1 (P-Thr36)
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
GSK3a (P-Ser21)
GSK3b (P-Ser9)
mTOR (P-Ser2448)
p27 (P-Thr198)
P53 (P-Ser15)
P70S6K (P-Thr421/Ser424)
PDK1 (P-Ser241)
  • Gene Symbol: PDK1 PDHK1
  • Gene ID: 5170
  • Accession #: O15530
PRAS40 (P-Thr246)
  • Gene Symbol: AKT1S1 PRAS40
  • Gene ID: 84335
  • Accession #: Q96B36
  • ELISA Kit: RayBio Human Phospho-PRAS40 (T246) ELISA
PTEN (P-Ser380)
Raf-1 (Ser301)
RPS6 (P-Ser235/Ser236)
RSK1 (P-Ser380)
RSK2 (P-Ser386)
  • Gene Symbol: RPS6KA3 ISPK1 MAPKAPK1B RSK2
  • Gene ID: 10432
  • Accession #: P51812

Kit Components

  • Human and Mouse AKT Pathway Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template
  • Manual

Additional Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Assay Procedure Summary

  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated anti-IgG
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Typical Data

NIH3T3 cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with PDGFβ for 10 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

293 cells were grown to 80% confluency and then serum starved 24 hours. Cells were either untreated (bottom panel) or treated (top panel) with Insulin for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

RayBiotech's AKT Pathway Phosphorylation Antibody Array was used to evaluate OLFML3's impact on ErbB-related signaling. Figure adapted from Zhu et al., J Nanobiotechnol, 2023.

RayBiotech's AKT Pathway Phosphorylation Antibody Array was used to assess the impact of SYK deletion on PI3K/AKT signaling in microglia from 5xFAD Alzheimer’s model mice. Figure adapted from Ennerfelt et al., Cell, 2022.

RayBiotech's AKT Pathway Phosphorylation Antibody Array was used to assess the effects of NLRP6 knockdown on PI3K/AKT signaling in glioblastoma cells. Figure adapted from Zhi et al., Nat Commun, 2023.

RayBiotech's AKT Pathway Phosphorylation Antibody Array was used to assess PI3K/AKT/mTOR activation in cervical epithelial lesions. Figure adapted from Li et al., Adv Sci, 2024.

RayBiotech's AKT Pathway Phosphorylation Antibody Array was used to assess exosomal miR-9's effects on PDK/AKT signaling in endothelial cells. Figure adapted from Lu et al., J Exp Clin Cancer Res, 2018.

RayBiotech's AKT Pathway Phosphorylation Antibody Array was used to evaluate EE02's impact on EGFR-driven signaling in MDA-MB-468 cells. Figure adapted from Li et al., J Exp Clin Cancer Res, 2019.

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

Datasheet (AAH-AKT-1.pdf)
SDS (AAH-AKT-1.pdf)
Excel (AAH-AKT-1_Analysis_Tool.xls)

citations

  • SYK coordinates neuroprotective microglial responses in neurodegenerative disease

    Year:
    2022
    Journal Name:
    Cell
    PubMed ID:
    36257314

  • SVEP1 is an endogenous ligand for the orphan receptor PEAR1

    Species:
    Human
    Sample Type:
    human umbilical vein endothelial cells (HUVECs)
    Year:
    2023
    Journal Name:
    Nature Communications

  • NLRP6 potentiates PI3K/AKT signalling by promoting autophagic degradation of p85α to drive tumorigenesis

    Species:
    Human
    Sample Type:
    HEK293T cell
    Year:
    2023
    Journal Name:
    Nature Communications

  • LIN28B-mediated PI3K/AKT pathway activation promotes metastasis in colorectal cancer models

    Year:
    2025
    Journal Name:
    Journal of Clinical Investigation
    PubMed ID:
    39808497

  • Activated mTOR Signaling in the RPE Drives EMT, Autophagy, and Metabolic Disruption, Resulting in AMD-Like Pathology in Mice

    Year:
    2025
    Journal Name:
    Aging Cell
    PubMed ID:
    39957408

  • Epithelial Genetic Muscarinic Receptor 3 Ablation Induces Sex-Specific Modulation of Colonic Intestinal Progenitor Cells and Response to Intestinal Injury

    Year:
    2025
    Journal Name:
    Journal of Crohns & Colitis
    PubMed ID:
    40052554

  • PIWIL2/PDK1 Axis Promotes the Progression of Cervical Epithelial Lesions via Metabolic Reprogramming to Maintain Tumor-Initiating Cell Stemness

    Year:
    2024
    Journal Name:
    Advanced science (Weinheim, Baden-Württemberg, Germany)
    PubMed ID:
    39499767

  • LAMA5-inspired adhesive dodecapeptide facilitates efficient dentine regeneration: An in vitro and in vivo study

    Species:
    Human
    Sample Type:
    human dental pulp cells (hDPCs)
    Year:
    2023
    Journal Name:
    International Endodontic Journal

  • Exosome-transmitted circCABIN1 promotes temozolomide resistance in glioblastoma via sustaining ErbB downstream signaling

    Species:
    Human
    Sample Type:
    LN229
    Year:
    2023
    Journal Name:
    Journal of nanobiotechnology

  • YBX1/lncRNA SBF2-AS1 interaction regulates proliferation and tamoxifen sensitivity via PI3K/AKT/MTOR signaling in breast cancer cells

    Species:
    Human
    Sample Type:
    MCF-7 cell
    Year:
    2023
    Journal Name:
    Molecular Biology Reports

You might also be interested by the following products:



Cat#
Description
Cond.
Price Bef. VAT
AAH-CYT-1-8
Human Cytokine Array C1
 8SampleKit