Human Receptor Array GS1 (Glass Slide)

Cat# GSH-REC-1-4

Size : 64SampleKit

Brand : RayBiotech

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Phone : +1 850 650 7790

Human Receptor Array GS1

G-Series Human Receptor Array 1 Kit. Semi-quantitative measurement of 40 human cytokines. Suitable for all liquid sample types.

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Product Description

Specifications

Size16 Sample Kit, 32 Sample Kit, 64 Sample Kit
SpeciesHuman
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeSemi-Quantitative
Number of Targets Detected40
Solid Support
Glass Slide
Design PrincipleSandwich-based
Method Of DetectionFluorescence Laser Scanner
Shipping TypeBlue ice
Storage-20°C

Introduction

RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 &mirco;L per array).

Product Features

  • High sensitivity (pg/mL to ng/mL levels)
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Test 16 samples on each slide
  • Data analysis software provides one-step computation

Target Names

Scroll over each target protein for more information
4-1BB
(TNFRSF9/CD137)
ALCAM
(CD166)
CD80
(B7-1)
BCMA
CD14
CD30
(TNFRSF8)
CD40 Ligand
(TNFSF5)
CEACAM-1
DR6
(TNFRSF21)
Dtk
Endoglin
(CD105)
ErbB3
E-Selectin
Fas
(TNFRSF6/Apo-1)
Flt-3 Ligand
GITR
(TNFRSF18)
HVEM
(TNFRSF14)
ICAM-3
(CD50)
Contactin-2
(TAG1)
IL-1 R1
IL-2 R gamma
(Common gamma Chain)
  • Gene Symbol: IL2RG
  • Gene ID: 3561
  • Accession #: P31785
IL-10 R beta
IL-17 RA
IL-21 R
LIMPII
Lipocalin-2
(NGAL)
L-Selectin
(CD62L)
LYVE-1
MICA
MICB
NRG1-beta 1
(HRG1-beta 1)
PDGF R beta
PECAM-1
RAGE
TIM-1
(KIM-1)
TRAIL R3
(TNFRSF10C)
Trappin-2
uPAR
VCAM-1
(CD106)
XEDAR
  • Gene Symbol: EDA2R TNFRSF27 XEDAR UNQ2448/PRO5727/PRO34080
  • Gene ID: 60401
  • Accession #: Q9HAV5

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Detection of Relative Protein Expression
  • Detecting Patterns of Cytokine Expression
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming a Biological Process
Kit Components
  • Antibody Array Slide/s
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with samples
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

GAL (GAL-QAH-REC-1.gal)
Excel (GSH-REC-1_Analysis_Tool.xlsx)

citations

  • Identification of a small-molecule targeting PLAGL2 DNA-binding domain inhibits extracellular matrix formation and enhances lenvatinib sensitivity in hepatocellular carcinoma

    Species:
    Human
    Sample Type:
    Serum (serum-free medium of Huh-7 cells)
    Year:
    2026
    Journal Name:
    Acta Pharmaceutica Sinica B
    PubMed ID:
    41909729
  • PFDN5 synergizes with anti-PD1 therapy to promote triple-negative breast cancer cell death through JAK2/STAT3/c-Myc signaling axis

    Species:
    Human
    Sample Type:
    Cell Lysate (cell lysates of TNBC cells); Tissue Lysate / Homogenate
    Year:
    2026
    Journal Name:
    Journal of Translational Medicine
    PubMed ID:
    42032729
  • hAECs restore follicular development in premature ovarian insufficiency via IGFBP2/IGF1R-mediated intercellular communication

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (concentrated hAEC conditioned medium)
    Year:
    2026
    Journal Name:
    Stem Cell Research & Therapy
    PubMed ID:
    42144654
  • Elevated LIF and JAK-STAT activation drive severe COVID-19 in myeloma patients receiving the BCMA-CD3 bispecific antibody Elranatamab

    Species:
    Human
    Sample Type:
    Plasma (myeloma patients with COVID-19)
    Year:
    2025
    Journal Name:
    Journal of Translational Medicine
    PubMed ID:
    39865264
  • Single-cell transcriptomics and epigenomics unravel the role of monocytes in neuroblastoma bone marrow metastasis

    Species:
    Human
    Sample Type:
    CLB-Ma cell, CHLA90 cell, peripheral blood mononuclear cells (PBMCs)
    Year:
    2023
    Journal Name:
    Nature Communications
  • Low-dose letrozole-HMG regimen reverses letrozole-induced endometrial impairment and improves frozen embryo transfer outcomes

    Species:
    Human
    Sample Type:
    Other Body Fluid (uterine fluid); Serum; Tissue Lysate / Homogenate (endometrial tissue extracts)
    Year:
    2025
    Journal Name:
    Frontiers in Cell and Developmental Biology
    PubMed ID:
    41476607
  • Cytokine Expression Profiling in Idiopathic Pulmonary Fibrosis: Insights From Integrative Proteomic Analysis

    Species:
    Human
    Sample Type:
    Tissue Lysate / Homogenate (IPF, control lung tissue lysates)
    Year:
    2025
    Journal Name:
    Canadian respiratory journal
    PubMed ID:
    41244106
  • Proteomic analysis identifies novel molecular signatures and immune-metabolic pathways in rheumatoid arthritis-associated interstitial lung disease

    Species:
    Human
    Sample Type:
    Serum (healthy, RA and RA-ILD pools)
    Year:
    2025
    Journal Name:
    Biomedical Reports
    PubMed ID:
    41159041
  • Serum cytokine profiles in children with IgA vasculitis with nephritis

    Species:
    Human
    Sample Type:
    Serum (children with IgAV and IgAVN)
    Year:
    2025
    Journal Name:
    Biomolecules & biomedicine
    PubMed ID:
    39235446
  • Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson’s disease models

    Species:
    Human
    Sample Type:
    human menstrual blood-derived endometrial stem cells (MenSCs)
    Year:
    2023
    Journal Name:
    Stem Cell Research & Therapy