IgG, F(ab')2 (FITC)

Cat# I1904-35F-2mg

Size : 2mg

Brand : US Biological

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I1904-35F IgG, F(ab')2 (FITC)

Clone Type
Polyclonal
Host
goat
Source
human
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA IF WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C (-20°C Glycerol)

Applications:
Suitable for use in FLISA and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Absorption Wavelength:
495nm

Emission Wavelength:
528nm

Fluorochrome/Protein Ratio:
3.0 moles FITC per mole of Goat IgG

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: human|Concentration: ~2mg/ml (UV280)|Form: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Labeled with fluorescein isothiocyanate (FITC).|Purity: Purified by immunoaffinity chromatography.|Immunogen: Human IgG F(ab’)2 fragment|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against Human IgG F(c).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Human IgG F(ab’)2 fragment
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by immunoaffinity chromatography.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against Human IgG F(c).
References
1. The and Feltkamp, Immunology, 18: 865, 1970.