IgG4 (MaxLight 550)

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Cat# I1904-89B1-ML550-100ul

Size : 100ul

Contact local distributor :

Phone : +1 850 650 7790

Brand : US Biological

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Contact local distributor :

Phone : +1 850 650 7790

I1904-89B1-ML550 IgG4 (MaxLight 550)

Clone Type

Monoclonal

Host

mouse

Source

human

Swiss Prot

P01861

Isotype

IgG1

Grade

Purified

Applications

FLISA IHC WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Applications:
Suitable for use in FLISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen and paraffin sections.
Optimal dilutions to be determined by the researcher.

Hybridoma:
SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: 3H2426 (HP6025)|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.|Purity: Purified|Immunogen: Purified human IgG4.|Specificity: Recognizes the heavy chain of human IgG4 at an epitope in the Fc region. Does not crossreact with IgG1, IgG2, IgG3, IgM or IgA. Species Crossreactivity: chimpanzee||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Purified human IgG4.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.

Purity

Purified

Specificity

Recognizes the heavy chain of human IgG4 at an epitope in the Fc region. Does not crossreact with IgG1, IgG2, IgG3, IgM or IgA. Species Crossreactivity: chimpanzee

References

1. Jefferis, R. et al. (1985). Immunology Letters. 10:223-252. 2. Agaimy, A. et al. (2010) Am J Surg Pathol. 34:271-8. 3. Strehl, J.D. et al. (2011) Numerous IgG4-positive plasma cells are ubiquitous in diverse localised non-specific chronic inflammatory conditions and need to be distinguished from IgG4-related systemic disorders. J Clin Pathol. Jan 12. 4. Black, C.M. et al. (1991) Immunology. 72:94-8. 5. Fernandez-Becerra, C. (2010) Malar J. 9:29. 6. Lee, H.W. et al. (2008) Korean J Parasitol. 46:195-8.