IgM (Biotin)

Cat# I1905-01B-2mg

Size : 2mg

Brand : US Biological

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Phone : +1 850 650 7790


I1905-01B IgM (Biotin)

Clone Type
Polyclonal
Host
goat
Source
mouse
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10,000-1:50,000
Immunohistochemistry: 1:1000-1:5000
Western Blot: 1:2000-1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: mouse|Concentration: ~2mg/ml (after reconstitution) |Form: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Mouse IgM mu heavy chain|Specificity: Recognizes mouse IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, mouse IgM and mouse serum. No reactivity was observed against other mouse heavy or light chain proteins.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Mouse IgM mu heavy chain
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, mouse IgM and mouse serum. No reactivity was observed against other mouse heavy or light chain proteins.
References
1. Bayer & Wilchek, Methods in Enzymology, 184: 138-160 (1990).