IL -17C Protein, Human, Recombinant (His & Avi), Biotinylated
Cat# TMPJ-00077-100ug
Size : 100ug
Brand : TargetMol
Contact local distributor :
Phone : +1 850 650 7790
IL -17C Protein, Human, Recombinant (His & Avi), Biotinylated
(Synonyms: interleukin-17C, interleukin 17C, IL-17CMGC126884, IL-17C, IL17C, Cytokine CX2, CX2) Copy Product InfoIL -17C Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The accession number is Q9P0M4.
For research use only—not for human use. No sales to individuals. Use as intended only.
User Guide of Recombinant Proteins
Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Biotinylated Human IL-17C, His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Human IL-17RE, hFc Tag with the EC50 of 4.5 ng/ml determined by ELISA (QC Test). |
| Description | IL -17C Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The accession number is Q9P0M4. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | Q9P0M4 |
| Amino Acid | His19-Val197 |
| Construction | His19-Val197 |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in 50mM MES (pH 6.0). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | interleukin-17C, interleukin 17C, IL-17CMGC126884, IL-17C, IL17C, Cytokine CX2, CX2 |
| Research Background | Interleukin 17C (IL 17C) is a 15 20 kDa glycosylated cytokine that plays an important role in mucosal immunity and chronic inflammation. IL 17C binds to IL 17 RE with high affinity and to IL 17 RA with low affinity. These two receptor chains can associate into a heterodimeric receptor for IL 17C. IL 17C expression is induced by inflammatory stimulation in colon and airway epithelial cells, keratinocytes, CD4+ T cells, macrophages, and dendritic cells. It is up regulated in various chronic inflammatory diseases including psoriasis, cystic fibrosis, and chronic obstructive pulmonary disease (COPD). IL 17 RE is reciprocally down regulated in psoriatic lesions. The interaction of IL 17C with IL 17 RE promotes mucosal immunity through the induction of anti bacterial peptides and pro inflammatory cytokines and chemokines. IL 17C action supports the integrity of the colon epithelium following infection induced damage but also contributes to psoriatic skin thickening and the progression of arthritis. IL 17C is additionally up regulated in Th17 cell dependent autoimmunity. In this setting, it exacerbates disease severity by inducing Th17 cell production of IL 17A, IL 17F, IL 22, CCR6, and CCL20. |
| Molecular Weight | 22.6 kDa (Predicted); 25-28 kDa (Reducing condition) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |