Insulin (IDDM2, ILPR, IRDN, MODY10, Proinsulin) (APC)

Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

Applications: 
Suitable for use in Immunofluorescence, ELISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.5-1ug/million cells 
Immunofluorescence: 1-2ug/ml 
Western Blot: 0.5-1ug/ml 
Immunoprecipitation: 1-2ug/500ug protein lysate
Immunohistochemistry: Frozen & Formalin-fixed: 0.5-1ug/ml for 30 minutes at RT
(No special pretreatment is required for staining of formalin/paraffin tissues.
Optimal dilutions to be determined by the researcher.

Positive Control:
Pancreas

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.

Note: Applications are based on unconjugated antibody.