Integrin alpha 5 beta 1 Protein, Human, Recombinant (Flag & His)

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Cat# NB-64-57277-20ug

Size : 20ug

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Phone : +1 850 650 7790

Brand : Neo Biotech

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Integrin alpha 5 beta 1 Protein, Human, Recombinant (Flag & His)

(Synonyms: VLA5A, VLA-5, integrin, α5 (fibronectin receptor, α polypeptide), integrin, alpha 5 (fibronectin receptor, alpha polypeptide), Integrin α5 β 1, Integrin alpha 5 β1, FNRA, CD49e) Copy Product Info

Synonyms: VLA5A, VLA-5, integrin, α5 (fibronectin receptor, α polypeptide), integrin, alpha 5 (fibronectin receptor, alpha polypeptide), Integrin α5 β 1, Integrin alpha 5 β1, FNRA, CD49e

Catalog No. TMPY-02159 Copy Product Info

Purity: 95.7%

Integrin alpha 5 beta 1 Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 192 kDa and the accession number is P08648&P05556-1.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:95.7%

Appearance:Lyophilized powder

Color:White

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	Integrin alpha 5 beta 1 Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 192 kDa and the accession number is P08648&P05556-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-Flag, C-His
Accession Number	P08648&P05556-1
Construction	A DNA sequence encoding the extracellular domain (Met 1-Tyr 995) of human ITGA5 (P08648) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 728) of human ITGB1 (P05556-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified Predicted N terminal: Phe 42 & Gln 21
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	VLA5A, VLA-5, integrin, α5 (fibronectin receptor, α polypeptide), integrin, alpha 5 (fibronectin receptor, alpha polypeptide), Integrin α5 β 1, Integrin alpha 5 β1, FNRA, CD49e

Chemical Properties

Molecular Weight	192 kDa (predicted); 120 &140 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

VLA5VLA 5integrin, α 5integrin, alpha 5integrin, a 5fibronectin receptor, α polypeptidefibronectin receptor, alpha polypeptidefibronectin receptor, a polypeptide

Related Tags: Integrin alpha 5 beta 1 Protein, Human, Recombinant (Flag & His) chemical structure | Integrin alpha 5 beta 1 Protein, Human, Recombinant (Flag & His) molecular weight