Reagents and instruments for immunology, cell biology and molecular biology.
 
> Integrin alpha 9 beta 1 (Integrin alpha 9, ITGA9, Integrin beta 1, ITGB1, Alpha-RLC, CD29, Fibronectin Receptor Subunit beta, FNRB, GPIIA, Integrin alpha-RLC, ITGA4L, MDF2, MSK12, RLC, VLA-4 Subunit beta, VLA-beta, VLAB) (APC)

Integrin alpha 9 beta 1 (Integrin alpha 9, ITGA9, Integrin beta 1, ITGB1, Alpha-RLC, CD29, Fibronectin Receptor Subunit beta, FNRB, GPIIA, Integrin alpha-RLC, ITGA4L, MDF2, MSK12, RLC, VLA-4 Subunit beta, VLA-beta, VLAB) (APC)

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Cat# I7661-36Z7-APC-100ul

Size : 100ul

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

I7661-36Z7-APCIntegrin alpha 9 beta 1 (Integrin alpha 9, ITGA9, Integrin beta 1, ITGB1, Alpha-RLC, CD29, Fibronectin Receptor Subunit beta, FNRB, GPIIA, Integrin alpha-RLC, ITGA4L, MDF2, MSK12, RLC, VLA-4 Subunit beta, VLA-beta, VLAB) (APC)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcehuman
Swiss ProtQ13797
IsotypeIgG1
GradeAffinity Purified
ApplicationsFC IP
CrossreactivityGp Hu Po
Shipping TempBlue Ice
Storage Temp4°C Do Not Freeze

Alpha9 integrin is a 140kD glycoprotein expressed as a heterodimer in association with the beta1 integrin. The alpha9 integrin is expressed by epithelial cells, including airway epithelial cells.

Applications:
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
SP2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 10D291 (Y9A2)|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Alpha9 integrin transfected lambda cells|Specificity: Recognizes human alpha9 integrin. Inhibits adhesion of alpha9 expressing cells to N-terminal osteopontin. Species crossreactivity: porcine and guinea pig||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Alpha9 integrin transfected lambda cells
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human alpha9 integrin. Inhibits adhesion of alpha9 expressing cells to N-terminal osteopontin. Species crossreactivity: porcine and guinea pig
References
1. Wang, A. et al. (1996) Differential regulation of airway epithelial integrins by growth factors. Am. J. Respir, Cell. Mol. Biol. 15: 664-672.2. Smith, L. L. et al. (1996) Osteopontin N-terminal domain contains a cryptic adhesive sequence recognised by alpha9 beta1 integrin. J. Biol. Chem. 271: 28485-28491. 3. Yokosaki, Y. et al. (1996) Differential effects of the integrins alpha9 beta1, alphaV/beta3 and alphaV beta6 on cell proliferative responses to tenascin. J. Biol. Chem. 271: 24144-24150.