Interleukin 1 Alpha (IL1a) BioAssay™ ELISA Kit (Goat)

Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Intended Use:
The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL1a in caprine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Sensitivity:
6.3pg/ml

Range:
15.6-1000pg/ml

Specificity:
This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Alpha (IL1a). No significant cross-reactivity or interference between Interleukin 1 Alpha (IL1a) and analogues was observed.

Precision:
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Test Principle:
The microplate provided in this kit has been pre-coated with an antibody specific to IL1a. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to IL1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL1a, biotin-conjugated antibody and
enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL1a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Kit Components:
96-well strip plate, 1x Plate
Standard, 2x vial
Detection Reagent A, 1×120ul
Detection Reagent B, 1×120ul
TMB Substrate, 1×9ml
Wash Buffer (30X), 1×20ml
Standard Diluent, 1×20ml
Assay Diluent A, 1×12ml
Assay Diluent B, 1×12ml
Stop Solution, 1×6ml

Storage and Stability:
Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.