Lambda Light Chain (Azide free) (HRP)
Cat# MBS6121528-5x0,2mL
Size : 5x0.2mL
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
Lambda light chain Antibody western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the Lambda light chain antibody detected the Lambda light chain protein (arrow).
Lambda light chain Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Lambda light chain Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Lambda Light Chain Secondary Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6121528Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two types of light chains… More details
Product Overview
Online Datasheet in PDFProduct Name
Lambda Light Chain, Secondary Antibody
Full Product Name
Lambda Light Chain (Azide free) (HRP)
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
HP6054
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein G Affinity Chromatography
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Applications Tested/Suitable for Lambda Light Chain secondary antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for Lambda Light Chain secondary antibody
ELISA: 1:1000
IHC: 1:10-50
WB: 1:500-16000
Applications are based on unconjugated antibody.
IHC: 1:10-50
WB: 1:500-16000
Applications are based on unconjugated antibody.
Immunohistochemistry (IHC) of Lambda Light Chain secondary antibody
Lambda light chain Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Lambda light chain Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Product References and Citations for Lambda Light Chain secondary antibody
58066: Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201 58067: Jefferis R, et al. Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol. Lett. 10: 223-252, 1985. PubMed: 3899923
Other Notes
Small volumes of Lambda Light Chain secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Lambda Light Chain purchase
MBS6121528 is a secondary antibody targeting Lambda Light Chain. Also known as Lambda Light Chain (Azide free) (HRP). Reactive in Human. Host: Mouse.
Additional Details
NCBI/Uniprot data below describe general gene information for Lambda Light Chain. It may not necessarily be applicable to this product.
NCBI Official Full Name
Ig lambda chain V-I region NEW
UniProt Protein Name
Ig lambda chain V-I region NEW
UniProt Entry Name
LV103_HUMAN
UniProt Comments for Lambda Light Chain
IGLV1-51: V segment of the variable region of immunoglobulins light chain that participates to the antigen recognition. Immunoglobulins (Igs), also known as antibodies, are membrane- bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors which, upon binding of a specific antigen (Ag), trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable region of one heavy chain, together with that of its associated light chain. Thus, each Ig has two antigen binding sites with remarkable affinity for a particular antigen. The variable regions are assembled by a process called V(D)J recombination and can then be subjected to somatic hypermutation after exposure to antigen to allow affinity maturation for a particular Ag (PubMed:20176268, PubMed:17576170). {ECO:0000303|PubMed:17576170, ECO:0000303|PubMed:20176268, ECO:0000303|PubMed:22158414}. Immunoglobulins are composed of two identical heavy chains and two identical light chains, linked by disulfide bonds. {ECO:0000303|PubMed:20176268}
Show more Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with Lambda Light Chain secondary antibody
| Reference Product | PubMed Publications |
|---|---|
| FLNA secondary antibody | >12 publications with Lambda Light Chain and FLNA |