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> Methyl linolenate [301-00-8]

Methyl linolenate [301-00-8]

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Cat# HY-21268-200mg

Size : 200mg

Brand : MedChemExpress

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Methyl linolenate is a polyunsaturated fattly acid (PUFA). Methyl linolenate has an anti-melanogenesis activity with an IC50 of 60 μM. Methyl linolenate can also be used for studies of the mechanisms and prevention of oxidation/peroxidation of unsaturated fatty acids.

For research use only. We do not sell to patients.

Methyl linolenate Chemical Structure

Methyl linolenate Chemical Structure

CAS No. : 301-00-8

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Other Forms of Methyl linolenate:

  • Methyl linolenate-13C18 Get quote
Description

Methyl linolenate is a polyunsaturated fattly acid (PUFA). Methyl linolenate has an anti-melanogenesis activity with an IC50 of 60 μM. Methyl linolenate can also be used for studies of the mechanisms and prevention of oxidation/peroxidation of unsaturated fatty acids[1][2][3][4].

In Vitro

Methyl linolenate (50, 100 μM, 48 h) significantly down-regulates microphthalmia-associated transcription factor (MITF) and tyrosinase-related proteins in B16F10 cells[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Methyl linolenate Related Antibodies
Molecular Weight

292.46

Formula

C19H32O2
CAS No.

301-00-8
Unlabeled CAS

Appearance

Liquid

Color

Colorless to light yellow

SMILES

CC/C=C\C/C=C\C/C=C\CCCCCCCC(OC)=O
Structure Classification
  • Others
Initial Source
  • Plants
  • other families
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (341.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4193 mL 17.0964 mL 34.1927 mL
5 mM 0.6839 mL 3.4193 mL 6.8385 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.68%

COA (237 KB) HNMR (191 KB) LCMS (106 KB)

Handling Instructions (2659 KB)
References

  • [1]. Huh S et al. Melanogenesis inhibitory effect of fatty acid alkyl esters isolated from Oxalis triangularis. Biol Pharm Bull, 2010, 33(7):1242-5.

    [2]. Li X et al. Benchmark Calculations for Bond Dissociation Enthalpies of Unsaturated Methyl Esters and the Bond Dissociation Enthalpies of Methyl Linolenate. J Phys Chem A, 2016, Jun 16, 120(23):4025-36  [Content Brief]

    [3]. Huh S, et al. Melanogenesis inhibitory effect of fatty acid alkyl esters isolated from Oxalis triangularis. Biol Pharm Bull. 2010;33(7):1242-5.  [Content Brief]

    [4]. Ko GA, et al. Sageretia thea fruit extracts rich in methyl linoleate and methyl linolenate downregulate melanogenesis via the Akt/GSK3β signaling pathway. Nutr Res Pract. 2018 Feb;12(1):3-12.  [Content Brief]

  • [1]. Huh S et al. Melanogenesis inhibitory effect of fatty acid alkyl esters isolated from Oxalis triangularis. Biol Pharm Bull, 2010, 33(7):1242-5.

    [2]. Li X et al. Benchmark Calculations for Bond Dissociation Enthalpies of Unsaturated Methyl Esters and the Bond Dissociation Enthalpies of Methyl Linolenate. J Phys Chem A, 2016, Jun 16, 120(23):4025-36

    [3]. Huh S, et al. Melanogenesis inhibitory effect of fatty acid alkyl esters isolated from Oxalis triangularis. Biol Pharm Bull. 2010;33(7):1242-5.

    [4]. Ko GA, et al. Sageretia thea fruit extracts rich in methyl linoleate and methyl linolenate downregulate melanogenesis via the Akt/GSK3β signaling pathway. Nutr Res Pract. 2018 Feb;12(1):3-12.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4193 mL 17.0964 mL 34.1927 mL 85.4818 mL
5 mM 0.6839 mL 3.4193 mL 6.8385 mL 17.0964 mL
10 mM 0.3419 mL 1.7096 mL 3.4193 mL 8.5482 mL
15 mM 0.2280 mL 1.1398 mL 2.2795 mL 5.6988 mL
20 mM 0.1710 mL 0.8548 mL 1.7096 mL 4.2741 mL
25 mM 0.1368 mL 0.6839 mL 1.3677 mL 3.4193 mL
30 mM 0.1140 mL 0.5699 mL 1.1398 mL 2.8494 mL
40 mM 0.0855 mL 0.4274 mL 0.8548 mL 2.1370 mL
50 mM 0.0684 mL 0.3419 mL 0.6839 mL 1.7096 mL
60 mM 0.0570 mL 0.2849 mL 0.5699 mL 1.4247 mL
80 mM 0.0427 mL 0.2137 mL 0.4274 mL 1.0685 mL
100 mM 0.0342 mL 0.1710 mL 0.3419 mL 0.8548 mL
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Methyl linolenate Related Classifications

Help & FAQs

Keywords:

Methyl linolenate301-00-8Linolenic acid methyl esterOthersPUFAanti-melanogenesisoxidationperoxidationInhibitorinhibitorinhibit

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