Micro NAD-Malate Dehydrogenase (NAD-MDH) Activity Assay Kit

Principle of the Assay: Malate dehydrogenase (MDH, EC1.1.1.37) exists widely in animals, plants, microorganisms and cultured cells. MDH in mitochondria is one of the key enzymes of tricarboxylic acid cycle (TCA), which catalyzes malate to oxaloacetic acid. On the contrary, MDH in cytoplasm catalyzes oxaloacetic acid to malate. Oxaloacetic acid is an important intermediate, which connects many important metabolic pathways. Therefore, MDH plays an important role in a variety of cellular physiological activities, including mitochondrial energy metabolism, malic acid-aspartic acid shuttle system, active oxygen metabolism and disease resistance. According to different coenzyme specificity, MDH can be divided into NAD-dependent MDH and NADP-dependent MDH. CheKine™ Micro NAD-Malate Dehydrogenase (NAD-MDH) Activity Assay Kit provides a simple, convenient and rapid NAD-MDH activity detection method, which is suitable for the detection of animal and plant tissues, cells, bacteria, serum (plasma) and other samples. The detection principle is that NAD-MDH catalyzes NADH to reduce oxaloacetic acid to malic acid, resulting in a decrease in light absorption at 340 nm.

Small volumes of NAD-MDH assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9719362 is an assay kit targeting NAD-MDH. Also known as Micro NAD-Malate Dehydrogenase (NAD-MDH) Activity Assay Kit.