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Monkeypox Virus A29L Protein Human IgA ELISA Kit Standard Curve
Monkeypox Virus A29L Protein Human IgA ELISA Kit
The Monkeypox Virus (MPXV) A29L Protein Human IgA ELISA Kit is an in vitro indirect ELISA for the semi-quantitative measurement of human IgA antibody against MPXV A29L protein in human serum or plasma.
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human, Monkeypox Virus (MPXV) |
| Detection Range | 1.37 Units/mL - 1000 Units/mL |
| Compatible Sample Types | Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 1,500-fold |
| Gene Symbols | IGA |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Indirect |
| Method Of Detection | Colorimetric |
| Research Area | Infectious Disease, Mpox |
| Shipping Type | Dry ice (additional shipping fee) |
| Storage | -80°C |
Introduction
This MPXVA29 human IgA antibody ELISA kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with the MPXVA29 protein, which combines with the corresponding antibody present in a sample and Positive Control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgA antibody is added, a complex form between biotinylated secondary antibody and human IgA antibody bound to the protein coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the anti-MPXVA29 IgA antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The Positive Control consists of an anti-MPXVA29 antibody. A biotinylated anti-Positive Control antibody is added to the Positive Control wells and treated identically to the samples. The intensity of the resulting color (measured at 450 nm) is used as a calibration curve for interpretation purposes in the different assays.
Kit Components
| Component | Size / Description |
|---|---|
| MPXV A29L Protein Coated 96-Well Microplate | 96 wells coated with MPXV A29L protein |
| Wash Buffer | 25 mL |
| Positive Control | 1 vial |
| Biotinylated Anti-Human IgA | 1 vial |
| Biotinylated Anti-Positive Control | 1 vial |
| HRP-Streptavidin | 1 vial |
| TMB One-Step Substrate Reagent | 12 mL |
| Stop Solution | 8 mL |
| Assay Diluent | 15 mL |
| Sample Diluent | 25 mL |
Additional Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed.
- Add 100 µL positive control, or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µL prepared Biotinylated Anti-Positive Control and Biotinylated Anti-Human IgA Antibody into respective wells. Incubate 1 hour at room temperature.
- Add 100 µL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 10 to 30 minutes at room temperature.
- Add 50 µL Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
