Monocarboxylate Transporter 8 (MCT8, XPCT)

Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and red blood cells use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain a high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboylic acid transporters (MCTs). At least 9 MCT related proteins have been identified in mammals that are expressed in a tissue specific manner.

Applications:
Suitable for use in Western Blot and ELISA. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1-5ug/ml using Chemiluminescence technique
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.

Control Peptide:
M4470-70B: Monocarboxylate Transporter 8, Human Control Peptide (MCT8, XPCT)

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.