mouse Anti-TCC (Terminal Complement Complex, MAC, Membrane Attack Complex, SC5b-9 Complex) (FITC) Monoclonal Antibody [Clone:aE11]
Cat# 298100-100ug
Size : 100ug
Brand : US Biological
Contact local distributor :
Phone : +1 850 650 7790
298100 TCC (Terminal Complement Complex, MAC, Membrane Attack Complex, SC5b-9 Complex) (FITC)
Clone Type
MonoclonalHost
mouseSource
humanIsotype
IgG2aGrade
Affinity PurifiedApplications
FC FLISA IF IHCCrossreactivity
Eq Hu PoShipping Temp
Blue IceStorage Temp
-20°CMonoclonal antibody TCC reacts with a C9 neoantigen of the terminal complement complex (TCC). The three distinct activation pathways of complement converge with the formation of a C5 convertase. The cleavage of C5 by this convertase initiates the lytic or terminal pathway. In contrast to the activation pathways, which require enzymatic cleavage for activation, the terminal pathway relies on conformational changes induced by binding. Binding of C6 facilitates binding of C7 which alters the conformation of the complex. After binding of C8, a variable number of C9 molecules associate with the C5b678 complex, which is also termed the terminal complement complex (TCC). The formation of TCC causes lysis of cells or can trigger a variety of cellular metabolic pathways resulting in the synthesis and release of inflammatory mediators. The TCC contains neoantigens that are absent from the individual native components. C9 neoantigens are present both in the membrane-bound (MAC) and the fluid-phase (SC5b-9) complex. TCC is present in normal human plasma and increased in patients with complement activation.
Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, FLISA and Immunohistochemistry. Not suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:
Flow Cytometry: 1:50
Immunohistochemistry (Frozen/Paraffin): 1:50
Immunofluorescence: 1;50. Frozen sections of 10um were dried, fixed in acetone at 4ºC for 10 minutes, dried and blocked for 20 minutes with PBSA.
Optimal dilutions to be determined by the researcher.
Positive Control:
Mucosa from patients with H. Pylori.
Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.