Mouse Anti-TET2 Monoclonal Antibody [Clone:CL6873]

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Cat# AMAb91439-25ul

Size : 25ul

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Phone : +1 850 650 7790

Brand : Atlas Antibodies

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Contact local distributor :

Phone : +1 850 650 7790

AMAb91439

Tet methylcytosine dioxygenase 2

Anti-TET2 Antibody

Monoclonal Antibody against HUMAN TET2

1.0 mg/ml

info@biovalley.fr

Immunohistochemistry analysis in human placenta and pancreas tissues using AMAb91439 antibody. Corresponding TET2 RNA-seq data are presented for the same tissues.

Product details

Target protein: Tet methylcytosine dioxygenase 2
Target gene: TET2
Verified species reactivity: Human
Interspecies information: Highest antigen sequence indentity to the following orthologs: RAT - ENSRNOG00000023579 (84%)MOUSE - ENSMUSG00000040943 (89%)
Clonality: Monoclonal
Clone ID: CL6873
Isotype: IgG2b
Host: Mouse
Buffer: The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Protein A purified
Notes: The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

Unit size: 100µl
Current lot: MAB-03660
Concentration: 1.0 mg/ml

Applications

Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 2-10 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:20 - 1:50
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 1 µg/ml

Validation

Immunofluorescence in Cell Lines (ICC-IF)
Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above)

Target information

Protein name: Tet methylcytosine dioxygenase 2
Gene name: TET2
Alternative gene names: FLJ20032, KIAA1546,
Ensembl:
UniProt:
Entrez:

Shipping and storage

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (2)

Fujikura K, Alruwaii ZI, Haffner MC, Trujillo MA, Roberts NJ, Hong SM, Macgregor-Das A, Goggins MG, Roy S, Meeker AK, Ding D, Wright M, He J, Hruban RH, Wood LDJ Pathol , 2021 May 21; 254(3):279-288. Epub 2021 May 212021 May 21
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Human Protein Atlas