Mouse CD38 Antibody : LE/AF
Cat# OASB00428
Size : 0.5mg
Brand : Aviva Systems Biology
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Phone : +1 850 650 7790
Mouse CD38 Antibody : LE/AF (OASB00428)
| Datasheets/Manuals | Printable datasheet for Mouse CD38 Antibody : LE/AF (OASB00428) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | NIMR-5 |
| Isotype | IgG2a |
| Host | Rat |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, IHC-F, IP, STIM, BL |
| Additional Information | Description: Murine CD38, a type II transmembrane glycoprotein, is a bifunctional ectoenzyme capable of catabolizing nicotinamide adenine dinucleotide (NAD+) to cyclic ADP-ribose (cADPR) and then hydrolyzing cADPR to adenosine diphosphoribose (ADPR). It is expressed at high levels on the surface of peripheral B-lineage cells and at low density on germinal center B cells from unimmunized mice. It has also been reported to be expressed at moderate levels on NK cells, a proportion of peripheral T cells, and a subpopulation of thymocytes which are mostly TCR gamma/delta+, CD4-, CD8-. Murine CD38 is also expressed by all Mac-1+ macrophages in the peritoneal cavities of unimmunized mice, but not by unstimulated bone-marrow-derived macrophages. Monoclonal antibodies to CD38 have been shown to induce B and T cell proliferation, protect B cells from apoptosis, and inhibit B lymphopoiesis. |
| Reconstitution and Storage | Store at -20C |
| Immunogen | BCL1 plasma membrane glycoproteins |
| Concentration | 0.5 mg/mL |
| Specificity | CD38 |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. Representative data are included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Fluorescein conjugate <= 1 ug/106 cells Biotin conjugate <= 1 ug/106 cells R-phycoerythrin conjugate <= 0.2 ug/106 cells Allophycocyanin conjugate <= 0.2 ug/106 cells Spectral Red conjugate <= 0.2 ug/106 cells |
| Application Info | Flow cytometry 9, Immunoprecipitation 1, Induction of B lymphocyte proliferation 5, Blocking 9 |
| Other Applications Data | Since applications vary, you should determine the optimum working dilution for the product that is appropriate for yourspecific need. |
| Other Applications Image 1 Data | Amount Used: 0.3 Ug/106 cells Cells from BALB/c spleen were double-stained with rat anti-mouse CD38-R-PE and rat anti-mouse CD19-FITC. Small lymphocytes were gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA). |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 in 2.0 mL of PBS/NaN3 anda stabilizing agent. Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Harada, N., L. Santos- Argumedo, R. Chang, J. C. Grimaldi, F. E. Lund, C. I. Brannan, N. G. Copeland, N. A. Jenkins, A. W. Heath, and R. M. . . Parkhouse. 1993. J. Immunol. 151:3111 2. Santos- Argumedo, L., C. Teixeira, G. Preece, P. A. Kirkham, and R. M. E. Parkhouse. 1993. J. Immunol. 151:3119 3. Howard, M., J. C. Grimaldi, J. F. Bazan, F. E. Lund, L. Santos- Argumendo, R. M. E. Parkhouse, T. F. Walseth, and H. C. Lee. 1993. Science 262:10564. Lund, F., N. Solvason, J. C. Grimaldi, R. M. Parkhouse, and M. Howard. 1995. Immunol. Today 16:469 5. Kirkham, P. A., L. Santos- Argumedo, M. M. Harnett, and R. M. Parkhouse. 1994. Immunology 83:513 6. Bean, A. G. D., D. I. Godfrey, W. G. Ferlin, L. Santos- Argumedo, R. M. E. Parkhouse, M. C. Howard, and A. Zlotnik. 1996. Int. Immunol. 7:213 7. Oliver, A. and J. Kearney. 1996. Personal Communication8. Kumagi, M., E. Coustan- Smith, D. J. Murray, O. Silvennoinen, K.G. Murti, W.E. Evans, F. Malavasi, and D. Campana. 1995. J. Exp. Med. 181:1101 9. Wykes, M.N., L. Beattie, G.G. Macpherson, and D.N. Hart. 2004. Immunology 113:318. |
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| Gene Symbol | CD38 |
| Gene Full Name | ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 |
| Alias Symbols | I-19, Cd38-r, ADPRC 1, Cd38-rs1 |
| NCBI Gene Id | 12494 |
| Protein Name | ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 |
| Description of Target | This gene encodes a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Knockout mice deficient for this gene display altered humoral immune responses. In addition, knockout mice exhibit higher locomotor activity and defects in nurturing and social behaviors. |
| Uniprot ID | P56528 |
| Protein Accession # | NP_031672.2 |
| Nucleotide Accession # | NM_007646.5 |
