Mouse CD79b/B29 Antibody : LE/AF
Cat# OASB01752
Size : 0.5mg
Brand : Aviva Systems Biology
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Mouse CD79b/B29 Antibody : LE/AF (OASB01752)
| Datasheets/Manuals | Printable datasheet for Mouse CD79b/B29 Antibody : LE/AF (OASB01752) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | HM79-12 |
| Isotype | IgG |
| Host | Hamster |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, IP, WB |
| Additional Information | Description: Murine CD79 is a disulfide-linked heterodimer composed of an alpha chain (CD79a) and a beta chain (CD79b) that associates non-covalently with membrane immunoglobulin (Ig) to form the B cell receptor (BCR) complex. Its expression is restricted to B lymphocytes first appearing on the surface at the pro-B cell stage prior to productive Ig gene rearrangements and remaining through all stages of B-cell differentiation prior to plasma cells. It has been proposed that the CD79 complex on pro-B cell surfaces may function to induce early B-cell differentiation. |
| Reconstitution and Storage | Store at -20C |
| Immunogen | CD79alpha/CD79beta heterodimers purified from mouse (BALB/c x NZB)F1 B cell lymphoma cells, WEHI231 |
| Concentration | 0.5 mg/mL |
| Specificity | CD79b |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conformto characteristics of a standard reference reagent. Representative data are included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified antibody <= 1 ug/106 cells Fluorescein conjugate <= 1 ug/106 cells Biotin conjugate <= 1 ug/106 cells R-phycoerythrin conjugate <= 0.2 ug/106 cells |
| Application Info | Flow cytometry 1, 2, Immunoprecipitation 1, Western blotting 1 |
| Other Applications Data | Since applications vary, you should determine the optimum working dilution for the product that is appropriate for yourspecific need. |
| Other Applications Image 1 Data | Amount Used: 1 Ug/106 cells BALB/c splenocytes were incubated with either hamster IgG (as an isotype control) or purified hamster anti-mouse CD79, and then stained with mouse anti-hamster IgG (H+L)-FITC. Small lymphocytes were gated and analyzed |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.0. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Koyama, M., K. Ishihara, H. Karasuyama, J.L. Cordell, A. Iwamoto, and T. Nakamura. 1997. Int. Immunol. 9:1767 2. Aviva Systems Biology, Inc. Unpublished observations. |
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| Gene Symbol | CD79B |
| Gene Full Name | B-cell antigen receptor complex-associated protein beta chain |
| Alias Symbols | Ig, B29, Igb, Igbe, Ig-be, Igbeta, Ig-beta |
| NCBI Gene Id | 15985 |
| Protein Name | B-cell antigen receptor complex-associated protein beta chain |
| Description of Target | The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Uniprot ID | P15530 |
| Protein Accession # | NP_001300868.1 |
| Nucleotide Accession # | NM_001313939.1 |
