G-Series Mouse Cytokine Array 1 Kit. Semi-quantitative measurement of 20 mouse cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 16 Sample Kit, 32 Sample Kit, 64 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 20 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.
This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).
This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).
Product Features
- High sensitivity (pg/mL to ng/mL levels)
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Test 16 samples on each slide
- Data analysis software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
GM-CSF
| IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-10
|
IL-12 p70
| IL-13
| IL-17A
| IL-2
| IL-3
|
IL-4
| IL-5
| IL-6
| IL-9
| KC (CXCL1)
|
MCP-1 (CCL2)
| M-CSF
| RANTES (CCL5)
| TNF alpha
| VEGF-A
|
Application Notes
Suggested Application
- Multiplexed Protein Detection
- Detection of Relative Protein Expression
- Detecting Patterns of Cytokine Expression
- Biomarker Screening
- Identifying Key Factors
- Confirming a Biological Process
Kit Components
- Antibody Array Slide/s
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- 30 ml Centrifuge Tube
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with samples
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAM-CYT-1.gal)
Excel (GSM-CYT-1_Analysis_Tool.xlsx)
citations
Targeting the Lipid Metabolism Proteins FASN and GPAM in Alveolar Type II Cells Decreases Lung Metastasis
- Species:
- Mouse
- Sample Type:
- Other / Unspecified (CtrlM, MCM samples)
- Year:
- 2026
- Journal Name:
- Cancer Discovery
- PubMed ID:
- 41778850
Dynamic changes in immune cell populations by AXL kinase targeting diminish liver inflammation and fibrosis in experimental MASH
- Species:
- Mouse
- Sample Type:
- Serum (MASH model mice)
- Year:
- 2024
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 38817615
Identifying overtraining biomarkers through proteomic analysis of extracellular vesicles derived from the central nervous system of male mice
- Species:
- Mouse
- Sample Type:
- Plasma (overtraining vs exercise vs sedentary)
- Year:
- 2025
- Journal Name:
- Physiological Reports
- PubMed ID:
- 41178061
Targeted Deletion of Peroxiredoxin 1 Enhances Anti-Tumor Immunity in Colorectal Cancer by Reprogramming the Immunosuppressive Tumor-Associated Macrophages
- Species:
- Mouse
- Sample Type:
- Other / Unspecified (macrophages BMDMs and RAW264.7 cells)
- Year:
- 2025
- Journal Name:
- MedComm
- PubMed ID:
- 41306557
Roxadustat alleviates nitroglycerin-induced migraine in mice by regulating HIF-1α/NF-κB/inflammation pathway
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Acta Pharmacologica Sinica
Induction of the Inflammasome Pathway by Tyrosine Kinase Inhibitors Provides an Actionable Therapeutic Target for Hepatocellular Carcinoma
- Species:
- Mouse
- Sample Type:
- Serum (HCC mice, TKI treatment)
- Year:
- 2024
- Journal Name:
- Cancers
- PubMed ID:
- 38672578
Repurposing of glatiramer acetate to treat cardiac ischemia in rodent models
- Year:
- 2024
- Journal Name:
- Nat Cardiovasc Res
- PubMed ID:
- 39215106
Mixed Th1/Th2/Th17 Responses Induced by Plant Oil Adjuvant-Based B. bronchiseptica Vaccine in Mice, with Mechanisms Unraveled by RNA-Seq, 16S rRNA and Metabolomics
- Species:
- Mouse
- Sample Type:
- Serum (vaccinated mice)
- Year:
- 2024
- Journal Name:
- Vaccines
- PubMed ID:
- 39460348



