Mouse Transforming Growth Factor Beta 3, TGFB3 ELISA Kit
Cat# E0854Mo-10X96wells
Size : 10X96wells
Brand : Bioassay Technology Laboratory
Product overview
Full product name
Transforming Growth Factor Beta 3
Code
E0854Mo
Assay type
Sandwich
Size
48T,96T
Sensitivity
2.51pg/ml
Detection range
5-1000pg/ml
Sample type
Serum, plasma, cell culture supernates
Species
Mouse
Storage
2-8ºC
Assay time
1h 30m
Background
Binds to TGF-beta. Could be involved in capturing and retaining TGF-beta for presentation to the signaling receptors (By similarity).
UniProt accession
MASS(Da)
93,829
GeneID
21814
Synonyms
Betaglycan;TGF-beta receptor type 3;TGF-beta receptor type III;TGFBR 3;TGFBR3;TGFR-3;Transforming growth factor beta receptor III;Transforming growth factor beta receptor type 3
Gene names
TGFBR3
Research area
Immunology
Target protein
TGFBR3
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard solution | 0.5ml x1 |
Standard diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop solution | 6ml x1 |
Substrate solution A | 6ml x1 |
Substrate solution B | 6ml x1 |
Wash buffer concentrate (25x) | 20ml x1 |
Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0854Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
640pg/ml | 2.288 | 2.157 | 2.101 |
2.027 | |||
320pg/ml | 1.39 | 1.376 | 1.319 |
1.362 | |||
160pg/ml | 0.881 | 0.877 | 0.82 |
0.873 | |||
80pg/ml | 0.548 | 0.548 | 0.491 |
0.547 | |||
40pg/ml | 0.297 | 0.29 | 0.234 |
0.283 | |||
0pg/ml | 0.068 | 0.056 | 0 |
0.045 |
Precision
We measured random samples of E0854Mo within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-Assay | 1 | 18 | 181.4 | 11.39 | 6.3 |
Intra-Assay | 2 | 18 | 450.9 | 19.83 | 4.4 |
Intra-Assay | 3 | 18 | 66.9 | 4.56 | 6.8 |
Protocols
Customer reviews and Q&As