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> MyoD1 (bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM) (HRP)

MyoD1 (bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM) (HRP)

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Cat# 168841-AF-HRP-100ul

Size : 100ul

Brand : US Biological

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Phone : +1 850 650 7790

168841-AF-HRPMyoD1 (bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM) (HRP)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcehuman
Swiss ProtP15172 (Human); P10085 (Mouse)
IsotypeIgG1,k
GradePurified
ApplicationsIHC
CrossreactivityCh Hu Mo Rt
Shipping TempBlue Ice
Storage Temp-20°C

Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm’s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing’s sarcomas and alveolar soft part sarcomas.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (formalin-fixed): 0.5-1ug/ml for 30 minutes at RT. Requires boiling tissue sections in 10mM Tris, 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
Optimal dilutions to be determined by the researcher.

Positive Control:
Rhabdomyosarcoma

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1,k|Clone No: 5.8A, MYD712|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).|Purity: Purified by Protein A/G affinity chromatography.|Immunogen: Recombinant mouse MyoD1 protein (5.8A) and Recombinant human MyoD1 protein (MYD712). Cellular Localization: Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentatiation.|Specificity: Recognizes the phosphor-protein, human MyoD1 at 45kD. Labels the nuclei of myoblasts in developing muscle tissues. Species Crossreactivity: mouse, rat, chicken. Does not react with myogenin, Myf5, or Myf6.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Recombinant mouse MyoD1 protein (5.8A) and Recombinant human MyoD1 protein (MYD712). Cellular Localization: Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentatiation.
Form
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes the phosphor-protein, human MyoD1 at 45kD. Labels the nuclei of myoblasts in developing muscle tissues. Species Crossreactivity: mouse, rat, chicken. Does not react with myogenin, Myf5, or Myf6.
References
1. Tallini G; Parham DM; Dias P; Cordon-Cardo C; Houghton PJ; Rosai J. Myogenic regulatory protein expression in adult soft tissue sarcomas. A sensitive and specific marker of skeletal muscle differentiation. American Journal of Pathology, 1994, 144(4):693-701. 2. Dias P; Parham DM; Shapiro DN; Tapscott SJ; Houghton PJ. Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility. Cancer Research, 1992 Dec 1, 52(23):6431-9. 3. Rosai J; Dias P; Parham DM; Shapiro DN; Houghton P. MyoD1 protein expression in alveolar soft part sarcoma as confirmatory evidence of its skeletal muscle nature. Am Journal of Surgical Pathology, 1991, 15(10):974-81.