MyoD1 (bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM) (AP)
Cat# MBS6123521-0,1mL
Size : 0.1mL
Brand : MyBiosource
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MyoD1 (bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM) (AP)
Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MyoD1 MAb (MYD712)
MyoD1 Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6123521MBS6123521 is an antibody targeting MyoD1. Also known as MyoD1 (bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM) (AP). Reactive in Chicken, Human,… More details
Product Overview
Online Datasheet in PDFProduct Name
MyoD1, Monoclonal Antibody
Full Product Name
MyoD1 (bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM) (AP)
Product Gene Name
anti-MyoD1 antibody
Product Synonym Names
bHLHc1; Class C basic helix-loop-helix protein 1; Myoblast determination protein 1; Myogenic differentiation 1; Myogenic factor 3 (Myf-3); Myogenin D1; PUM
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
MYD712
Host
Mouse
Species Reactivity
Chicken, Human, Mouse, Rat
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Specificity
Recognizes human MyoD1. Species Crossreactivity: mouse, rat, and chicken. Others-not known.
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Molecular Weight
34,501 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (17741110..17743678). Location: 11p15.4
OMIM
159970
Immunogen
Recombinant human MyoD1 protein. Cellular Localization: Nuclear
Conjugate
AP
Positive Control
Rhabdomyosarcoma
Product Categories/Family for anti-MyoD1 antibody
Antibodies; Transcription Factors
Applications Tested/Suitable for anti-MyoD1 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen/Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-MyoD1 antibody
IHC-F/P: 0.5-1ug/ml for 30 minutes at RT
IP: 0.5-1ug/500ug protein lysate
IF: 0.5-1ug/ml
WB: 0.25-0.5ug/ml
Applications are based on unconjugated antibody.
IP: 0.5-1ug/500ug protein lysate
IF: 0.5-1ug/ml
WB: 0.25-0.5ug/ml
Applications are based on unconjugated antibody.
Product References and Citations for anti-MyoD1 antibody
1. Tallini G; Parham DM; Dias P; Cordon-Cardo C; Houghton PJ; Rosai J. Myogenic regulatory protein expression in adult soft tissue sarcomas. A sensitive and specific marker of skeletal muscle differentiation. American Journal of Pathology, 1994, 144(4):693-701. 2. Dias P; Parham DM; Shapiro DN; Tapscott SJ; Houghton PJ. Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility. Cancer Research, 1992 Dec 1, 52(23):6431-9. 3. Rosai J; Dias P; Parham DM; Shapiro DN; Houghton P. MyoD1 protein expression in alveolar soft part sarcoma as confirmatory evidence of its skeletal muscle nature. Am Journal of Surgical Pathology, 1991, 15(10):974-81.
Other Notes
Small volumes of anti-MyoD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for MyoD1. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
myoblast determination protein 1
NCBI Official Synonym Full Names
myogenic differentiation 1
NCBI Official Symbol
MYOD1
NCBI Official Synonym Symbols
PUM; MYF3; MYOD; bHLHc1
UniProt Protein Name
Myoblast determination protein 1
UniProt Synonym Protein Names
Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3
Protein Family
Myoblast determination protein
UniProt Gene Name
MYOD1
UniProt Synonym Gene Names
BHLHC1; MYF3; MYOD; bHLHc1; Myf-3
UniProt Entry Name
MYOD1_HUMAN
NCBI Protein Information
myoblast determination protein 1; myf-3; myogenic factor 3; class C basic helix-loop-helix protein 1
NCBI Summary for MyoD1
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for MyoD1
MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.
Protein type: DNA-binding; RNA splicing; Transcription factor
Chromosomal Location of Human Ortholog: 11p15.4
Cellular Component: nucleoplasm; transcription factor complex; myofibril; nuclear chromatin; cytoplasm; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; nuclear hormone receptor binding
Biological Process: transcription from RNA polymerase II promoter; muscle development; skeletal muscle development; skeletal muscle fiber adaptation; positive regulation of skeletal muscle regeneration; regulation of RNA splicing; myotube differentiation involved in skeletal muscle regeneration; muscle cell fate commitment; skeletal muscle fiber development; myotube cell development; cellular response to starvation; protein amino acid phosphorylation; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; myoblast cell fate determination; regulation of alternative nuclear mRNA splicing, via spliceosome
Show more Protein type: DNA-binding; RNA splicing; Transcription factor
Chromosomal Location of Human Ortholog: 11p15.4
Cellular Component: nucleoplasm; transcription factor complex; myofibril; nuclear chromatin; cytoplasm; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; nuclear hormone receptor binding
Biological Process: transcription from RNA polymerase II promoter; muscle development; skeletal muscle development; skeletal muscle fiber adaptation; positive regulation of skeletal muscle regeneration; regulation of RNA splicing; myotube differentiation involved in skeletal muscle regeneration; muscle cell fate commitment; skeletal muscle fiber development; myotube cell development; cellular response to starvation; protein amino acid phosphorylation; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; myoblast cell fate determination; regulation of alternative nuclear mRNA splicing, via spliceosome
Research Articles on MyoD1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-MyoD1 antibody
| Reference Product | PubMed Publications |
|---|---|
| JUN antibody | >66 publications with MyoD1 and JUN |
| TCF3 antibody | >12 publications with MyoD1 and TCF3 |
| ID1 antibody | >8 publications with MyoD1 and ID1 |
| SRF antibody | >7 publications with MyoD1 and SRF |
| RB1 antibody | >6 publications with MyoD1 and RB1 |
| HDAC1 antibody | >5 publications with MyoD1 and HDAC1 |
| SP1 antibody | >5 publications with MyoD1 and SP1 |
| CDK4 antibody | >4 publications with MyoD1 and CDK4 |
| SMAD3 antibody | >4 publications with MyoD1 and SMAD3 |
| MEF2A antibody | >4 publications with MyoD1 and MEF2A |
Pathways associated with anti-MyoD1 antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| C-MYB Transcription Factor Network Pathway antibodies | C-MYB Transcription Factor Network Pathway Diagram |
| CDO In Myogenesis Pathway antibodies | CDO In Myogenesis Pathway Diagram |
| Developmental Biology Pathway antibodies | Developmental Biology Pathway Diagram |
| Id Signaling Pathway antibodies | Id Signaling Pathway Diagram |
| Myogenesis Pathway antibodies | Myogenesis Pathway Diagram |
| Notch-mediated HES/HEY Network Pathway antibodies | Notch-mediated HES/HEY Network Pathway Diagram |
| Regulation Of Nuclear SMAD2/3 Signaling Pathway antibodies | Regulation Of Nuclear SMAD2/3 Signaling Pathway Diagram |
| Regulation Of Retinoblastoma Protein Pathway antibodies | Regulation Of Retinoblastoma Protein Pathway Diagram |
| Signaling Events Mediated By HDAC Class III Pathway antibodies | Signaling Events Mediated By HDAC Class III Pathway Diagram |
| MiRs In Muscle Cell Differentiation Pathway antibodies | MiRs In Muscle Cell Differentiation Pathway Diagram |
Diseases associated with anti-MyoD1 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms Antibodies | >202 publications with MyoD1 and Neoplasms |
| Nervous System Diseases Antibodies | >76 publications with MyoD1 and Nervous System Diseases |
| Disease Models, Animal Antibodies | >27 publications with MyoD1 and Disease Models, Animal |
| Necrosis Antibodies | >26 publications with MyoD1 and Necrosis |
| Muscular Atrophy Antibodies | >16 publications with MyoD1 and Muscular Atrophy |
| Inflammation Antibodies | >14 publications with MyoD1 and Inflammation |
| Breast Neoplasms Antibodies | >11 publications with MyoD1 and Breast Neoplasms |
| Lung Neoplasms Antibodies | >11 publications with MyoD1 and Lung Neoplasms |
| Adenocarcinoma Antibodies | >10 publications with MyoD1 and Adenocarcinoma |
| Heart Diseases Antibodies | >10 publications with MyoD1 and Heart Diseases |
Organs/Tissues associated with anti-MyoD1 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Muscle Antibodies | >603 publications with MyoD1 and Muscle |
| Heart Antibodies | >38 publications with MyoD1 and Heart |
| Eye Antibodies | >10 publications with MyoD1 and Eye |
| Lymph Antibodies | >8 publications with MyoD1 and Lymph |
| Placenta Antibodies | >2 publications with MyoD1 and Placenta |