Myogenin (Class C Basic Helix-loop-helix Protein 3, bHLHc3, Myogenic Factor 4, Myf-4, MYOG, BHLHC3, MYF4)
Cat# M9790-05C-100ug
Size : 100ug
Brand : US Biological
Contact local distributor :
Phone : +1 850 650 7790
M9790-05CMyogenin (Class C Basic Helix-loop-helix Protein 3, bHLHc3, Myogenic Factor 4, Myf-4, MYOG, BHLHC3, MYF4) discontinued, see M9790-05A
Specifications
Myogenin, also called MYF4, is a 34kD, 224aa basic-HLH transcription factor member of the myogenic regulatory factor (MRF) family. It induces fibroblastic differentiation to myoblasts and is expressed most highly early in the skeletal muscle differentiation process. Within the region used as an immunogen, human myogenin shares 93-91aa identity with mouse and rat myogenin, respectively. Isoforms may exist that diverge at aa155 (246aa form) or that are truncated at aa215.
Applications:
Suitable for use in Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Immunocytochemistry: 8-25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.