Nonyl Acridine Orange (NAO)
Cat# 70012
Size : 50MG
Brand : Biotium
Contact local distributor :
Phone : +1 850 650 7790
Nonyl Acridine Orange (NAO)[75168-11-5]
Cellular Stains
Troubleshooting Tips for Fluorescence Staining
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst
Tech Tip: Fluorescence Bleed-Through and Cross-Talk
Product Description
NAO is a green fluorescent mitochondrial dye whose staining is not dependent on mitochondrial membrane potential (1), unlike JC-1 (70011) or Rhodamine 123 (70010). NAO has been used to study multidrug resistance (2) and to measure changes in mitochondrial mass during apoptosis (3). The dye is reported to bind cardiolipin, a phosphoprotein in the inner mitochondrial membrane (4). Also see our MitoView™ Dyes and other mitochondrial dyes.
- λEx/λEm (MeOH) = 495/522 nm
- ε (MeOH) = 63,000
- Orange solid soluble in DMSO or DMF
- Store at 4°C and protect from light, especially in solution
- C26H38BrN3
- MW: 473
- [75168-11-5]
Technology
Tech Tips
Product Attributes
CAS number
75168-11-5
Size
50 mg
Probe cellular localization
Mitochondria
For live or fixed cells
For live/intact cells
Assay type/options
No-wash staining, Real-time imaging
Potential dependence
Mitochondrial potential-independent
Colors
Red
Excitation/Emission
495/522 nm
Documents, Protocols, SDS and COA
Documents
COA
Request the Certificate of AnalysisReferences
1. Cytometry 9, 206 (1988).
2. Cancer Res 51, 4665 (1991).
3. Exp Cell Res 214, 323 (1994).
4. FEBS Letters 260, 236 (1990).
Citations
1. Cytometry 9, 206 (1988).
2. Cancer Res 51, 4665 (1991).
3. Exp Cell Res 214, 323 (1994).
4. FEBS Letters 260, 236 (1990).
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