Size : 5mg
Phone : +1 850 650 7790
Synonyms:
| Description | Orcein is a natural dye derived from lichen species, composed of a mixture of phenazone compounds that bind to chromatin through electrostatic and hydrophobic interactions at acidic pH. For fluorescence microscopy, orcein solutions exhibit emission peaks at 587 nm at low concentrations and 620 nm at high concentrations, with stained chromatin showing an emission peak at 585 nm and a shoulder at 620 nm. In light microscopy, acetic orcein remains a standard method for visualizing polytene chromosome banding in Drosophila salivary glands, enabling high-resolution analysis of interphase chromatin structure after endoreplication. Additionally, orcein selectively stains elastic fibers and has been used to evaluate fibrosis quality in liver tissue, where its staining pattern helps distinguish progressive from regressive fibrosis. |
| In vivo | Orcein is used in mouse models for histochemical staining of elastic fibers in atherosclerotic plaques. In apoE/LDLR-/- mice, orcein combined with martius scarlet blue (OMSB) staining enables qualitative and quantitative analyses of brachiocephalic artery sections, identifying elastic fibers (purple), collagen (blue), erythrocytes (yellow), and fibrin (red). The method is simple, under 1 hour, and compatible with automated stainers for digital segmentation. |
| Molecular Weight | 500.507 |
| Formula | C28H24N2O7 |
| Cas No. | 1400-62-0 |
| Smiles | CC1=C(Nc2c(C)cc(O)cc2O)C(=O)C=c2oc3=CC(=O)C(Nc4c(C)cc(O)cc4O)=C(C)c3c12 |
| Relative Density. | 1.31g/cm3 |
| Storage | Keep away from direct sunlight, Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO: Soluble |
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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