The Permeabilization and Blocking Buffer (5X) is a concentrated stock solution for one step cell permeabilization and blocking. It is designed for use during immunofluorescence staining for fluorescence microscopy.

• Permeabilizes fixed cells for immunofluorescence staining
• Blocks non-specific binding of antibodies
• Can be used to dilute antibodies for staining, and as a wash buffer
• Contains goat serum, not for use with anti-goat or anti-sheep antibodies
• 100 mL of 5X solution

Note: this buffer contains goat serum and therefore is not compatible with anti-goat and anti-sheep secondary antibodies. We recommend Permeabilization Buffer (22016) and 10% Fish Gelatin Blocking Agent (22010) for use with anti-goat and anti-sheep secondary antibodies.