Reagents and instruments for immunology, cell biology and molecular biology.
 
> Ponceau S [6226-79-5]

Ponceau S [6226-79-5]

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Cat# HY-12489-500mg

Size : 500mg

Brand : MedChemExpress

Contact local distributor :


Phone : +1 850 650 7790

Description

Ponceau S (Acid Red 112) is a non-specific protein dye commonly used as a stain for Western blot. Ponceau S is used in an acidic aqueous solution that is compatible with antibody-antigen binding and dyes the proteins on the membrane red[1][2][3].

In Vitro

Concentration of Ponceau S (0.001-2 (w/v); 5-10 min), acid concentration and acid type (acetic acid, trichloroacetic acid/sulfosalicylic acid) doesn’t affect the sensitivity of protein detection[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ponceau S Related Antibodies

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (131.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 35.71 mg/mL (46.95 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3148 mL 6.5740 mL 13.1480 mL
5 mM 0.2630 mL 1.3148 mL 2.6296 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References