Protease Inhibitor Cocktail (100X in DMSO, EDTA plus)
Cat# K1019-1
Size : 1mlA+1mlB
Brand : APExBIO Technology
Contact local distributor :
Phone : +1 850 650 7790
This product is a protease inhibitor cocktail (100X in DMSO, EDTA plus), specifically designed for use during cell lysis and protein extraction processes. It is a ready-to-use concentrated solution that can inhibit a variety of endogenous proteases. Also included is a separate 0.5 M EDTA solution, which effectively inhibits metalloproteases.
The inhibitor cocktail consists of six carefully optimized broad-spectrum protease inhibitors dissolved in DMSO, capable of efficiently inhibiting serine proteases, cysteine proteases, aspartic proteases, and aminopeptidases commonly present in cell lysates. EDTA is provided for inhibition of metalloproteases. If immobilized metal affinity chromatography (IMAC) is involved for protein purification, or if two-dimensional (2D) gel electrophoresis analysis is planned, EDTA must be thoroughly removed by extensive dialysis or desalting prior to purification.
Specification: 1 Set equals 1X (1 mL A + 1 mL B), 10 Sets equals 10X (1 mL A + 1 mL B), and so on.
- 1. Haonan Meng, Muhammad Jalal, et al. "17-AAG promotes the degradation of HSP90 client METTL3 to suppress MYC RNA m6A modification and expression in colorectal cancer." International Journal of Biological Macromolecules Volume 337, Part 1, January 2026, 149421
- 2. Xingchen Dong, Yaoting Zhang, et al. "TP53-dependent antitumor effects of DHODH Inhibition in nasopharyngeal carcinoma." Discov Oncol. 2025 Nov 30;17(1):14. PMID: 41318884
- 3. Ye Li, Jian Li, et al. "Catalytic-state structure of Candidatus Hydrogenedentes Cas12b revealed by cryo-EM studies." Nucleic Acids Res. 2025 Jun 20;53(12):gkaf519. PMID: 40539514
- 4. Meng-Wei Ko, Ao Mei, et al. "Osteoclast-expanded supercharged NK cells perform superior antitumour effector functions." BMJ Oncol. 2025 Jun 10;4(1):e000676. PMID: 40510443
- 5. Caiying Liu, Furong Gao, et al. "Extracellular GMFB activates the non - canonical FAS - FAF1 pathway to induce lysosomal dysfunction in early diabetic retinopathy." Int J Biol Macromol. 2025 Jul;318(Pt 1):145082. PMID: 40490177
- 6. Ning Wang, Dandan Zhou, et al. "Iron Homeostasis-Regulated Adaptive Metabolism of PEGylated Ultrasmall Iron Oxide Nanoparticles." ACS Nano. 2025 Apr 8;19(13):13381-13398. PMID: 40135968
- 7. Hunter N Bomba, Melody D Fulton, et al. "A Unique Prodrug Targeting the Prostate-Specific Membrane Antigen for the Delivery of Monomethyl Auristatin E." Bioconjug Chem. 2025 Feb 19;36(2):169-178. PMID: 39881600
- 8. Ruiqi Qiu, Mingzhu Yang, et al. "AAV2-PDE6B restores retinal structure and function in the retinal degeneration 10 mouse model of retinitis pigmentosa by promoting phototransduction and inhibiting apoptosis." Neural Regen Res. 2025 Aug 1;20(8):2408-2419. PMID: 39359097
- 9. Min Shao, Haipeng Cheng, et al. "Abnormal mitochondrial iron metabolism damages alveolar type II epithelial cells involved in bleomycin-induced pulmonary fibrosis." Theranostics. 2024 Apr 15;14(7):2687-2705. PMID: 38773980
- 10. Liu L, Yin S, et al. "TRAF6 Promotes PRMT5 Activity in a Ubiquitination-Dependent Manner." Cancers, 2023, 15(9): 2501. PMID: 37173967
- 11. Yu Z, Wu Z, et al. "Structural basis of a two-step tRNA recognition mechanism for plastid glycyl-tRNA synthetase." Nucleic Acids Research, 2023, 51(8): 4000-4011. PMID: 36912079
- 12. Reham Alsaadi. "Characterization of post-translational modification of ATG16L1 in antibacterial autophagy." University of Ottawa.2019
| Thaw on ice, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying. |
| Applications: WB, Co-IP, pull-down, IF, IHC, Flow Cytometry, kinase assay and etc. |
| Tube | Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| A (100X in DMSO) | ||||||
| A2573 | AEBSF.HCl | Serine protease inhibitor | Proteases|Serine Protease | 30827-99-7 | C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl | |
| A2574 | Aprotinin | Inhibitor of bovine pancreatic trypsin | Proteases|Serine Protease | 9087-70-1 | ||
| A2575 | Bestatin | Aminopeptidase inhibitor | Proteases|Aminopeptidase | 58970-76-6 | CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O | |
| A2576 | E-64 | Cysteine protease inhibitor,irreversible | Proteases|Cathepsin | 66701-25-5 | CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O | |
| A2570 | Leupeptin | Inhibitor of serine and cysteine proteases | Proteases|Serine Protease | 103476-89-7 | O=C(C(C(10)(10)C(C(10)(10)10)(10)C(10)(10)10)(10)N(10)C(C(10)(10)10)=O)N(10)C(C(N(10)C(C(10)=O)(10)C(10)(10)C(10)(10)C(10)(10)/N=C(N(10)10)/N(10)10)=O)(10)C(10)(10)C(C(10)(10)10)(10)C(10)(10)10 | |
| A2571 | Pepstatin A | Aspartic proteinases inhibitor | Proteases|Other Proteases | 26305-03-3 | CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O | |
| B (100X in ddH2O) | ||||||
| EDTA, disodium salt, dihydrate | Metalloprotease inhibitor | protease | 6381-92-6 | |||
| Stored at -20°C, and stable for at least 12 months. | ||||||