Protein Phosphatase 2A, alpha (PP2Aa) (FITC)
Cat# MBS6495546-0,1mL
Size : 0.1mL
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
WB: 3T3 cell lysate was probed with anti-PP2A, A subunit (0.5ug/ml).
Protein Phosphatase 2a, Alpha (PP2Aa) Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6495546MBS6495546 is an antibody targeting PP2Aa. Also known as Protein Phosphatase 2A, alpha (PP2Aa) (FITC). Reactive in Human, Mouse, Porcine, Rat, Xenopus. Host: Rabbit. More details
Product Overview
Online Datasheet in PDFProduct Name
Protein Phosphatase 2A, alpha (PP2Aa), Polyclonal Antibody
Full Product Name
Protein Phosphatase 2A, alpha (PP2Aa) (FITC)
Product Gene Name
anti-PP2Aa antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Porcine, Rat, Xenopus
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Recognizes PP2A, A subunit, Mr 63-65kD. Species Crossreactivity: Human. Predicted to crossreact with mouse, rat, porcine and Xenopus based on sequence homology.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Matching Pairs
Antibody: PP2Aa (MBS6495546)
Positive Control: PP2Aa (MBS657179)
Positive Control: PP2Aa (MBS657179)
Molecular Weight
65,309 Da
OMIM
605983
Immunogen
Synthetic peptide of 13 amino acids (MAAADGDDSLY-Ahx-C), containing amino acids 1-11 of human PP2A, A subunit. Ahx is aminohexanoic acid (KLH-conjugated).
Conjugate
FITC
Note
Preservative Free
Product Categories/Family for anti-PP2Aa antibody
Antibodies; Enzymes; Phosphatase
Applications Tested/Suitable for anti-PP2Aa antibody
Western Blot (WB)
Application Notes for anti-PP2Aa antibody
WB: 0.5-2ug/ml dilution detects PP2A, A subunit in RIPA lysates from EGF-stimulated A431 cell lysate. NIH 3T3 cell lysate was resolved by electrophoresis, transferred to nitro-cellulose and probed with anti-PP2A, A subunit). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Band is PP2A, A subunit (63-65kD).
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Other Notes
Small volumes of anti-PP2Aa antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for PP2Aa. It may not necessarily be applicable to this product.
NCBI Official Full Name
serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
NCBI Official Synonym Full Names
protein phosphatase 2 regulatory subunit A, alpha
NCBI Official Symbol
PPP2R1A
NCBI Official Synonym Symbols
MRD36; PR65A; PP2AAALPHA; PP2A-Aalpha
UniProt Protein Name
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
UniProt Synonym Protein Names
Medium tumor antigen-associated 61 kDa protein; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha
UniProt Gene Name
PPP2R1A
UniProt Entry Name
2AAA_HUMAN
NCBI Protein Information
serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
UniProt Comments for PP2Aa
PPP2R1A: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with TP53 and SGOL1. Interacts with PLA2G16; this interaction might decrease PP2A activity. Belongs to the phosphatase 2A regulatory subunit A family.
Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit
Chromosomal Location of Human Ortholog: 19q13.41
Cellular Component: chromosome, pericentric region; cytosol; protein phosphatase type 2A complex
Molecular Function: antigen binding; protein binding; protein heterodimerization activity; protein phosphatase regulator activity
Biological Process: apoptosis; chromosome segregation; G2/M transition of mitotic cell cycle; mitotic nuclear envelope reassembly; mRNA catabolic process, nonsense-mediated decay; protein amino acid dephosphorylation; protein complex assembly
Disease: Mental Retardation, Autosomal Dominant 36
Show more Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit
Chromosomal Location of Human Ortholog: 19q13.41
Cellular Component: chromosome, pericentric region; cytosol; protein phosphatase type 2A complex
Molecular Function: antigen binding; protein binding; protein heterodimerization activity; protein phosphatase regulator activity
Biological Process: apoptosis; chromosome segregation; G2/M transition of mitotic cell cycle; mitotic nuclear envelope reassembly; mRNA catabolic process, nonsense-mediated decay; protein amino acid dephosphorylation; protein complex assembly
Disease: Mental Retardation, Autosomal Dominant 36
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with anti-PP2Aa antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| AMER1 Mutants Destabilize The Destruction Complex Pathway antibodies | AMER1 Mutants Destabilize The Destruction Complex Pathway Diagram |
| AMPK Signaling Pathway antibodies | AMPK Signaling Pathway Diagram |
| AMPK Signaling Pathway antibodies | AMPK Signaling Pathway Diagram |
| APC Truncation Mutants Have Impaired AXIN Binding Pathway antibodies | APC Truncation Mutants Have Impaired AXIN Binding Pathway Diagram |
| ARMS-mediated Activation Pathway antibodies | ARMS-mediated Activation Pathway Diagram |
| AXIN Missense Mutants Destabilize The Destruction Complex Pathway antibodies | AXIN Missense Mutants Destabilize The Destruction Complex Pathway Diagram |
| AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway antibodies | AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway Diagram |
| Activated TLR4 Signalling Pathway antibodies | Activated TLR4 Signalling Pathway Diagram |
| Adaptive Immune System Pathway antibodies | Adaptive Immune System Pathway Diagram |
| Adrenergic Signaling In Cardiomyocytes Pathway antibodies | Adrenergic Signaling In Cardiomyocytes Pathway Diagram |
Diseases associated with anti-PP2Aa antibody
| Disease Name | Pubmed Publications |
|---|---|
| Disease Progression Antibodies | >5 publications with PP2Aa and Disease Progression |
| Death Antibodies | >3 publications with PP2Aa and Death |
| Nervous System Diseases Antibodies | >2 publications with PP2Aa and Nervous System Diseases |
| Inflammation Antibodies | >2 publications with PP2Aa and Inflammation |
| Fibrosis Antibodies | >2 publications with PP2Aa and Fibrosis |
| Heart Diseases Antibodies | >1 publications with PP2Aa and Heart Diseases |
| Weight Loss Antibodies | >1 publications with PP2Aa and Weight Loss |
| Kidney Diseases Antibodies | >1 publications with PP2Aa and Kidney Diseases |
| Liver Diseases Antibodies | >1 publications with PP2Aa and Liver Diseases |
| Osteoarthritis Antibodies | >1 publications with PP2Aa and Osteoarthritis |
Organs/Tissues associated with anti-PP2Aa antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Ovary Antibodies | >8 publications with PP2Aa and Ovary |
| Lung Antibodies | >6 publications with PP2Aa and Lung |
| Blood Antibodies | >4 publications with PP2Aa and Blood |
| Prostate Antibodies | >3 publications with PP2Aa and Prostate |
| Liver Antibodies | >3 publications with PP2Aa and Liver |
| Brain Antibodies | >3 publications with PP2Aa and Brain |
| Muscle Antibodies | >2 publications with PP2Aa and Muscle |
| Lymph Node Antibodies | >2 publications with PP2Aa and Lymph Node |
| Lymph Antibodies | >2 publications with PP2Aa and Lymph |
| Stomach Antibodies | >1 publications with PP2Aa and Stomach |