Rabbit Anti-ACADVL Polyclonal Antibody

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Cat# HPA019006-25ul

Size : 25ul

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Phone : +1 850 650 7790

Brand : Atlas Antibodies

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Contact local distributor :

Phone : +1 850 650 7790

HPA019006

acyl-CoA dehydrogenase, very long chain

Anti-ACADVL Antibody

Polyclonal Antibody against HUMAN ACADVL

0.1 mg/ml

info@biovalley.fr

Immunohistochemistry analysis in human duodenum and lymph node tissues using HPA019006 antibody. Corresponding ACADVL RNA-seq data are presented for the same tissues.

Product details

Target protein: acyl-CoA dehydrogenase, very long chain
Target gene: ACADVL
Verified species reactivity: Human, Mouse, Rat
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000018574 (85%)RAT - ENSRNOG00000018114 (86%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

Unit size: 100µl
Current lot: 000003482
Concentration: 0.1 mg/ml

Applications

Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:50 - 1:200
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Validation

Immunofluorescence in Cell Lines (ICC-IF)
Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated against independent antibody
Western Blot (WB)Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated in Western blot using relevant lysates (see Western blot application images above)

Target information

Protein name: acyl-CoA dehydrogenase, very long chain
Gene name: ACADVL
Alternative gene names: ACAD6, LCACD, VLCAD,
Ensembl:
Entrez:
UniProt:

Shipping and storage

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (1)

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Human Protein Atlas