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> Rabbit Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains), conjugated with FITC Antibody

Rabbit Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains), conjugated with FITC Antibody

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Cat# orb21627-2ml

Size : 2ml

Brand : Biorbyt

Contact local distributor :


Phone : +1 850 650 7790

Rabbit anti Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains) Antibody (FITC)

SKU: orb21627
This product can be custom made

Description

Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to rat IgG, heavy and light chains. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immuno- fluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Research Area

Immunology & Inflammation

Images & Validation

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Rabbit anti Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains) Antibody (FITC)
ReactivityRat

Key Properties

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Antibody TypeSecondary Antibody
HostRabbit
ClonalityPolyclonal
ConjugationFITC

Storage & Handling

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StorageThe lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Form/AppearanceFITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added IgG protein concentration 10 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.3. No foreign prot
Expiration Date12 months from date of receipt.
DisclaimerFor research use only
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Protocol Information