Quantibody® Rat Inflammation Array 1 Kit. Detects 10 Rat Inflammatory Factors. Suitable for all liquid sample types.
Rat Inflammation Array Q1
Rat Inflammation Array Q1
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Rat |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 10 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Inflammation, JAK/STAT Signaling, MAPK Signaling, NFkB Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
| IL-4
|
IL-6
| IL-10
| IL-13
| MCP-1 (CCL2)
| TNF alpha
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Rat Inflammation Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAR-INF-1.gal)
Excel (QAR-INF-1_Analysis_Tool.xls)
citations
Pregnancy-induced differential expression of SARS-CoV-2 and influenza a viral entry factors in the lower respiratory tract
- Species:
- Rat
- Sample Type:
- bronchoalveolar lavage (BAL) fluid, lung
- Year:
- 2023
- Journal Name:
- Plos One
Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson’s disease models
- Species:
- Rat
- Sample Type:
- cerebrospinal fluid (CSF), serum
- Year:
- 2023
- Journal Name:
- Stem Cell Research & Therapy
Skin-targeted delivery of extracellular vesicle-encapsulated curcumin using dissolvable microneedle arrays
- Species:
- Rat
- Sample Type:
- skin tissue
- Year:
- 2022
- Journal Name:
- Acta Biomaterialia
Anti-Inflammatory and Antiarrhythmic Effects of Beta Blocker in a Rat Model of Rheumatoid Arthritis
- Species:
- Rat
- Sample Type:
- Cell Lysate (Heart tissue)
- Year:
- 2020
- Journal Name:
- Journal of the American Heart Association
Pathological presentation of cardiac mitochondria in a rat model for chronic kidney disease
- Species:
- Rat
- Sample Type:
- Plasma (Chronic Kidney Disease model)
- Year:
- 2018
- Journal Name:
- Plos One
- PubMed ID:
- 29889834
Bilateral activation of STAT3 by phosphorylation at the tyrosine-705 (Y705) and serine-727 (S727) positions and its nuclear translocation in primary sensory neurons following unilateral sciatic nerve injury
- Species:
- Rat
- Sample Type:
- CSF (puncture of the cisterna magna)
- Year:
- 2018
HMGB1 Turns Renal Tubular Epithelial Cells into Inflammatory Promoters by Interacting with TLR4 During Sepsis
- Species:
- Rat
- Sample Type:
- Conditioned Media (Tubular epithelial cells treated with TLR4)
- Year:
- 2016
- PubMed ID:
- 26312770
The monoacylglycerol lipase inhibitor JZL184 decreases inflammatory response in skeletal muscle contusion in rats
- Species:
- Rat
- Sample Type:
- Serum (Serum ); Tissue Lysate (Sprague dawlet rats with skeletal muscle contusion injury. Muscle lysates)
- Year:
- 2015
- Journal Name:
- European Journal of Pharmacology
- PubMed ID:
- 25912803
APRIL promotes proliferation, secretion and invasion of fibroblast-like synoviocyte from rats with adjuvant induced arthritis
- Species:
- Rat
- Sample Type:
- Conditioned Media (Folicular like Synoviocytes in culture)
- Year:
- 2015
- Journal Name:
- Molecular Immunology
- PubMed ID:
- 25466610
CB2R orchestrates fibrogenesis through regulation of inflammatory response during the repair of skeletal muscle contusion
- Species:
- Rat
- Sample Type:
- Tissue Lysate (Skeletal Muscle tissue lysate, lower calf)
- Year:
- 2015
- Journal Name:
- International Journal of Clinical and Experimental Pathology
- PubMed ID:
- 26097533