Cytometric bead array (CBA) designed to quantitatively measure 13 human proteins involved in cell death pathways using flow cytometry. Optimized for serum, plasma, and cell culture supernatant.
RayPlex® Human Cell Death Bead Array (13-Plex)
RayPlex® Human Cell Death Bead Array (13-Plex)
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Product Description
Specifications
| Size | 48 tests, 96 tests, 192 tests, 480 tests |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 13 |
| Solid Support | Fluorescent Microbeads |
| Design Principle | Sandwich-based |
| Method Of Detection | Flow Cytometry |
| Research Area | Necrosis |
| Shipping Type | Blue ice |
| Storage | Mixed storage. See datasheet. |
Product Features
- Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
- 4 hour processing time
- 5-10x more cost-effective than ELISA
- Compatible with most flow cytometers
- No specialized or dedicated instrument(s) needed
- High specificity and system reproducibility
- Well-suited for high throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| Bcl-2
| BID
| CD40 (TNFRSF5)
| CD40 Ligand (TNFSF5)
| |
| Fas (TNFRSF6/Apo-1)
| Fas Ligand (TNFSF6)
| TNF alpha
| TNF beta (TNFSF1B)
| TNF RI (TNFRSF1A)
|
| TNF RII (TNFRSF1B)
| TRAIL R1 (TNFRSF10A/DR4)
| TRAIL R2 (TNFRSF10B/DR5)
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- RayPlex™ Multiplex Bead Cocktail
- 1X Assay Diluent
- 20X Wash Buffer
- RayBio® Serum/Plasma Diluent
- Lyophilized Protein Standard Mix
- 1X Biotinylated Detection Antibody Cocktail
- 10X PE Conjugate
- Flow Cytometer Setup Bead Cocktail
- V-bottom 96-well Microplate
- Filter 96-well Microplate
Other Materials Required
- Orbital 96-well plate shaker (with ability to reach 1000 rpm)
- Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
- Aluminum foil or 96-well Plate Aluminum Sealers
- Distilled water
- 1.5 ml polypropylene microcentrifuge tubes or similar
- Microcentrifuge
- If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
- Optional: 96-well round bottom plate (to prepare samples)
- Optional: High throughput sampler (HTS) for 96-well plate reading
Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!
Protocol Outline
- Prepare Reagents, Standards, and Samples
- Add RayPlex™ Multiplex Bead Cocktail to each well
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluorophore
- Analyze with a compatible flow cytometer
- Perform data analysis
Storage/Stability
Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.