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> Recombinant Human Glycoprotein A33

Recombinant Human Glycoprotein A33

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Cat# 230-30101-200

Size : 200ug

Brand : RayBiotech

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Phone : +1 850 650 7790

Recombinant Human Glycoprotein A33

Recombinant Human Glycoprotein A33

Recombinant Human Glycoprotein A33

Recombinant human glycoprotein A33 (transmembrane), derived from transfected human HEK293 cells.
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Product Description

Specifications

Size10 µg, 50 µg, 200 µg
SpeciesHuman
Accession Number
Q99795
Gene Symbols
GPA33
Protein Name / Synonyms
Glycoprotein A33, Glycoprotein A33 (Transmembrane), Transmembrane Glycoprotein A33, Transmembrane Glycoprotein A33, A33
Expressed Region
Ile22-Val235
Expression SystemHEK293 cells
TagN-terminal histidine tag
Purity
>95%
Purity Determined BySDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)
Recombinant human GPA33 protein has a calculated molecular mass of 24 kDa. Due to the abundant glycosylation, it migrates as approximately 30-35 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Format
Lyophilized powder
Formulation
Lyophilized from a 0.2 µm filtered solution in PBS
Concentration (lot specific)
Determined by Pierce BCA protein assay kit
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
Shipping TypeAmbient temperature
Storage-20°C
SDS-PAGE Image
SDS-PAGE
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions
Lane 3: Treated protein with deglycosylation enzymes under native conditions
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.

Stability & Storage

Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.

Custom Labeling Request

We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.