Recombinant human interferon, gamma with an N-terminal His-tag, derived from the transfected human HEK293 cells.
Figure 1. Deglycosylation analysis of purified recombinant proteins. Lane 1, protein ladder (kDa). Lane 2, glycosylated protein (delivered to customers). Lanes 3 and 4, deglycosylated proteins under native and denature conditions respectively.
Recombinant Human Interferon gamma, IFNG Protein
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Product Description
Specifications
| Size | 100 µg |
| Species | Human |
| Accession Number | P01579 |
| Gene Symbols | IFNG |
| Protein Name / Synonyms | Interferon gamma, IFN-gamma, Immune interferon |
| Expressed Region | Gln24-Gln166 |
| Expression System | HEK293 cells |
| Tag | N-terminal histidine tag |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 17 kDa. Due to the abundant glycosylation, it migrates as approximately 20-25 kDa major protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4) with 1% mannitol and 5% trehalose |
| Concentration (lot specific) | Determined by Pierce BCA protein assay |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Endotoxin Level | <0.5 EU per µg of the protein as determined by the LAL method |
| Research Area | Cancer, Inflammation, Autophagy |
| Recombinant or Native | Recombinant |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
| Storage / Stability | Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles. |
SDS-PAGE Image
Activity
Decreasing cell viability significantly. Measured in a cytotoxicity assay using HT-29 human colon cancer cells in a dose-dependent manner.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.