Recombinant Human TNF ALPHA
Recombinant Human TNF ALPHA
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Product Description
Specifications
| Size | 50 µg |
| Species | Human |
| Protein Name / Synonyms | TNF ALPHA, |
| Purity | >98% by SDS PAGE and HPLC analysis |
| Molecular Weight (kDa) | 17.4kD (157 Amino acid sequence) |
| Format | Purified recombinant cytokine - lyophilized |
| Concentration (lot specific) | Total protein concentration ~0.1 mg/ml after reconstitution |
| Preservative | None present |
| Recommended Applications | ELISA (0.2-0.4ng/well), Functional Assays (0.05-20.0ng/ml), Western Blotting (1.5-3.0ng/lane), ELISA (0.2-0.4ng/well), Functional Assays (0.05-20.0ng/ml), Western Blotting (1.5-3.0ng/lane) |
| Endotoxin Level | <0.1ng/ug |
| Research Area | Cardiovascular Disease, Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
229-20151 is an E. coli-derived recombinant protein corresponding to the C-terminal 157 amino acids of human Tumour Necrosis Factor Alpha (TNFa), a cytokine which exerts cytotoxic effects on a wide range of tunour cells.
229-20151 has an ED50 of <0.05 ng/ml as determined by an assay measuring cytolysis of L929 cells in the presence of Actinomycin D.
229-20151 has an ED50 of <0.05 ng/ml as determined by an assay measuring cytolysis of L929 cells in the presence of Actinomycin D.
Specifications
Size
50 ug
Assay Buffers
TRIS buffered saline
Stability & Storage
Storage/Stability
Prior to reconstitution store at +4°C. Following reconstitution store at -20°C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution