Recombinant Monkeypox virus (MPXV) envelope protein A28 homolog with C-terminal His-tag, derived from the transfected human HEK293 cells.
Recombinant Monkeypox Virus Envelope protein A28 homolog, A30L
Recombinant Monkeypox Virus Envelope protein A28 homolog, A30L
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Product Description
Specifications
| Size | 100 µg |
| Species | Monkeypox virus (MPXV) |
| Accession Number | Q8V4U9 |
| Gene Symbols | A30L |
| Expressed Region | Gln22-Leu146 |
| Expression System | HEK293 cells |
| Tag | C-terminal His-tag |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of ∼14 kDa. Due to the abundant glycosylation, it migrates as approximately ∼20-30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4) with 1% mannitol and 5% trehalose |
| Concentration (lot specific) | Determined by BCA protein assay |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water. |
| Endotoxin Level | <0.5 EU per µg of the protein as determined by the LAL method |
| Research Area | Infectious Disease, Mpox |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE:
Lane 1: protein standard ladder (kDa).
Lane 2: untreated protein.
Lane 3: treated protein with deglycosylation enzymes under native conditions.
Lane 4: treated protein with deglycosylation enzymes under denature conditions.
Stability & Storage
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.