Recombinant Monkeypox Virus IFN-alpha/beta-receptor-like Secreted Glycoprotein B16R

Cat# 230-30236-100

Size : 100ug

Brand : RayBiotech

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Phone : +1 850 650 7790

Recombinant Monkeypox Virus IFN-alpha/beta-receptor-like Secreted Glycoprotein B16R

Recombinant Monkeypox virus IFN-alpha/beta-receptor-like secreted glycoprotein (B16R) with a C-terminal His-tag, derived from the transfected HEK293 cells.
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Product Description

Specifications

Size100 µg
SpeciesMonkeypox Virus (MPXV)
Accession Number
Q8V4R2
Gene Symbols
B16R
Expressed Region
His21-Glu352
Expression SystemHEK293 cells
TagC-terminal His-tag
Purity
>90%
Purity Determined BySDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)
Recombinant protein product has a calculated molecular mass of ∼38 kDa. Due to the abundant glycosylation, it migrates as approximately ∼50 Da protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Format
Liquid
Formulation
Filtered solution in PBS with 1% mannitol and 5% trehalose
Concentration (lot specific)
Determined by BCA protein assay
Endotoxin Level<0.5 EU per µg of the protein as determined by the LAL method
Research AreaInfectious Disease, Mpox
Shipping TypeBlue ice
Storage-20°C
SDS-PAGE
SDS-PAGE
Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: protein standard ladder (kDa).
Lane 2: untreated protein.
Lane 3: treated protein with deglycosylation enzymes under native conditions.
Lane 4: treated protein with deglycosylation enzymes under denature conditions.

Stability & Storage

Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

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