Recombinant Mouse GM-CSF

GranulocyteMacrophage Colony Stimulating Factor is a 22 kD, pleiotropic cytokine that is a white blood cell growth factor. It controls the production and function of blood cells by stimulating stem cells to produce granulocytes and monocytes. GMCSF differs from GCSF in that it affects more cell types including macrophages and eosinophils. Moreover, GMCSF is part of the immune/inflammatory cascade, a process crucial for fighting infection. Interestingly, GMCSF expression may have pathological implications. Autocrine expression of GMCSF in myeloid leukemia cells is suspected to play a role in neoplasia, the formation of a new and abnormal growth of tissue. Additionally, GMCSF expression has also been documented in certain solid tumors. There have also been reports of GMCSF in synovial fluid from patients with arthritis suggesting that GMCSF may play a role in tissue damage associated with the inflammatory process. Blocking GMCSF is thought to have therapeutic potential by reducing inflammation. Some drugs are currently being developed to block GMCSF.

The biological activity of Mouse GMCSF was determined by proliferation of the factordependent murine cell line, DA3 (Ihle, J.N. et al., 1984, Advances in Viral Oncology, G. Klein, ed. Raven Press, New York, NY. 4:95 137). The expected ED₅₀ is 10 60 pg/ml.

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Met

Lyophilized

The predicted molecular weight of Recombinant Mouse GMCSF is Mr 14.3 kDa. However, the actual molecular weight as observed by migration on SDSPAGE is 14 kDa (reducing conditions).

14.3

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at 20°C to 70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at 20°C to 70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Parker, MW. et al. (2008) Cell 134: 496
2. Whitsett, JA. et al. (2002) Annual Review of Physiology 64: 775