Respiratory Syncytial Virus (RSV) Antibody, IgG, BioAssay™ ELISA Kit (Mouse)

The Respiratory Syncytial Virus (RSV) Antibody, IgG, BioAssay™ ELISA Kit is for the qualitative determination of mouse RSV concentrations in serum.

Principle of the Assay:
This ELISA Kit employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with RSV antigen. Samples are pipetted into the wells with mouse anti-mouse IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of mouse anti-respiratory syncytial virus (RSV) antibody (IgG) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Specificity:
This assay has high sensitivity and excellent specificity for detection of mouse anti-respiratory syncytial virus (RSV) antibody (IgG). No significant crossreactivity or interference between mouse anti-respiratory syncytial virus (RSV) antibody (IgG) and analogues was observed.

Precision:
Intra-assay Precision: CV <15%
Inter-assay Precision: CV <15%

Kit Components:
227896A: Microtiter Strips, 1x48 wells or 1x96 wells
227896B: Negative Control, 1x500ul or 1x1ml
227896C: Positive Control, 1x500ul or 1x1ml
227896D: Sample Diluent, 1x6ml or 1x12ml
227896E: IgG (HRP), 1x6ml or 1x12ml
227896F: Wash Buffer, 20X, 1x25ml or1x50ml
227896G: Substrate A, 1x3ml or 1x6ml
227896H: Substrate B, 1x3ml or 1x6ml
227896J: Stop Solution, 1x3ml or 1x16ml

Note: The Stop Solution (227896J) provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability:
Store all kit components at 4°C. Stable for 6 months after receipt. Once opened, the kit is stable for 2 weeks providing this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.