SHF siRNA (Mouse)
Cat# MBS828132-30nmol
Size : 30nmol
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
SHF siRNA – SH2 Domain-Containing Adapter Protein F (Mouse)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828132MBS828132 is an siRNA targeting SHF. Also known as SHF siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit SHF expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
SHF, siRNA
Full Product Name
SHF siRNA (Mouse)
Product Gene Name
SHF sirna
Product Synonym Names
SH2 domain-containing adapter protein F
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
SHF siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
26,263 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SHF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for SHF sirna
RNA Interference (RNAi)
Other Notes
Small volumes of SHF sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for SHF. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
SH2 domain-containing adapter protein F
NCBI Official Synonym Full Names
Src homology 2 domain containing F
NCBI Official Symbol
Shf
UniProt Protein Name
SH2 domain-containing adapter protein F
Protein Family
Protein shifted
UniProt Gene Name
Shf
UniProt Entry Name
SHF_MOUSE
NCBI Protein Information
SH2 domain-containing adapter protein F
UniProt Comments for SHF
SHF: Adapter protein which may play a role in the regulation of apoptosis in response to PDGF. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function
Biological Process: apoptosis
Protein type: Unknown function
Biological Process: apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with SHF sirna
| Reference Product | PubMed Publications |
|---|---|
| Src sirna | >4 publications with SHF and Src |
| Fgfr1 sirna | >1 publications with SHF and Fgfr1 |
| Alk sirna | >1 publications with SHF and Alk |
Diseases associated with SHF sirna
| Disease Name | Pubmed Publications |
|---|---|
| Heart Failure Antibodies | >124 publications with SHF and Heart Failure |
| Vascular Diseases Antibodies | >30 publications with SHF and Vascular Diseases |
| Heart Defects, Congenital Antibodies | >29 publications with SHF and Heart Defects, Congenital |
| Fibrosis Antibodies | >22 publications with SHF and Fibrosis |
| Disease Models, Animal Antibodies | >19 publications with SHF and Disease Models, Animal |
| Bone Diseases Antibodies | >17 publications with SHF and Bone Diseases |
| Kidney Diseases Antibodies | >15 publications with SHF and Kidney Diseases |
| Inflammation Antibodies | >13 publications with SHF and Inflammation |
| Hypertrophy Antibodies | >11 publications with SHF and Hypertrophy |
| Arrhythmias, Cardiac Antibodies | >9 publications with SHF and Arrhythmias, Cardiac |
Organs/Tissues associated with SHF sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Heart Antibodies | >208 publications with SHF and Heart |
| Bone Antibodies | >39 publications with SHF and Bone |
| Liver Antibodies | >38 publications with SHF and Liver |
| Brain Antibodies | >36 publications with SHF and Brain |
| Embryonic Tissue Antibodies | >36 publications with SHF and Embryonic Tissue |
| Lung Antibodies | >9 publications with SHF and Lung |
| Intestine Antibodies | >5 publications with SHF and Intestine |
| Eye Antibodies | >3 publications with SHF and Eye |
| Ovary Antibodies | >2 publications with SHF and Ovary |
| Pituitary Gland Antibodies | >2 publications with SHF and Pituitary Gland |