Small Nuclear Ribonucleoprotein 70kDa, Human Recombinant
Small Nuclear Ribonucleoprotein 70kDa, Human Recombinant
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Product Description
Specifications
| Size | 20 µg, 1000 µg |
| Species | Human |
| Accession Number | P08621 |
| Gene Id | 6625 |
| Protein Name / Synonyms | U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. |
| Expression System | E. coli |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Format | Sterile Filtered clear solution. |
| Formulation | U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA and 0.02% SDS. |
| Recommended Applications | Western-Blot with monoclonal anti-hexa-His-tag antibody & anti SNRP70 autoantibody positive sample. |
| Research Area | Cancer, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein (lacking 66 internal amino acids outside the known epitope-containing areas) fused to a hexahistidine purification tag. The Calculated molecular weight is 45.0 kDa (U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight and the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis).
Coating Concentration
0.3-0.6 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.
Immunological Functions
- Binds IgG-type human auto-antibodies.
- Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Stability & Storage
Storage/Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.