TCP1-alpha Antibody
Cat# OASE00325
Size : 100ug
Brand : Aviva Systems Biology
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Phone : +1 850 650 7790
TCP1 alpha-alpha Antibody (OASE00325)
| Datasheets/Manuals | Printable datasheet for TCP1 alpha-alpha Antibody (OASE00325) |
|---|---|
| COA Datasheet | 1 µg/ml of SMC-479 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody. |
| Predicted Species Reactivity | Bovine|Caenorhabditis elegans|Dog|Drosophila|Drosophila melanogaster|Guinea Pig|Hamster|Human|Monkey|Mouse|Porcine|Rabbit|Rat|Saccharomyces cerevisiae|Yeast |
|---|---|
| Clonality | Monoclonal |
| Clone | 91a |
| Isotype | IgG2a |
| Host | Rat |
| Conjugation | Unconjugated |
| Application | FC,ICC,IF,IP,WB |
| Additional Information | Background Info: Detects a 60kDa protein corresponding to the molecular weight of TCP1 alpha; also detects a molecular mass band of ~92kDa. Cross reactivity with human Hsp60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome. |
| :: | Scientific Background: T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60,Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL. |
| :: | Certificate of Analysis: 1ug/mL was sufficient for detection of TCP1 alpha in 20 ug of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody. |
| Reconstitution and Storage | -20°C |
| Immunogen | Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region). |
| Purification | Protein G Purified |
| Concentration | 1 mg/ml |
| Specificity | Detects ~60 & ~92kDa. Cross reactivity with human HSP60 in WB. Reacts weakly with S. cerevisiae, consistent with the epitope being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recog TCP1 of P. sativum, and the sequence of A. thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome. |
| Dilution | WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
| Storage Buffer | PBS pH7.4, 50% glycerol, 0.1% sodium azide |
| Description | T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL. |
| Gene Symbol | Tcp1 |
|---|---|
| Gene Full Name | t-complex protein 1 |
| Alias Symbols | AI528772, c-c, c-cpn, CCT, CCT-alpha, Cct1, Ccta, p6, p63, t-complex polypeptide 1, T-complex protein 1 subunit alpha, T-complex protein 1 subunit alpha A, T-complex protein 1 subunit alpha B, tailless complex polypeptide 1A, tailless complex polypeptide 1B, Tcp-, Tcp-1, TCP-1-A, TCP-1-alpha, TCP-1-B, Tp6, Tp63, TR, TRic. |
| NCBI Gene Id | 21454 |
| Protein Name | T-complex protein 1 subunit alpha|T-complex protein 1 subunit alpha isoform 1 [Mus musculus] |
| Description of Target | Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin (By similarity). |
| Uniprot ID | P11983 |
| Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_038714.2 |
