Orb1088240-48 | THB(Tetrahydrobiopterin) ELISA Kit Biovalley

THB(Tetrahydrobiopterin) ELISA Kit

Catalog Number	orb1088240
Category	Assays and Kits
Description	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Tetrahydrobiopterin (THB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tetrahydrobiopterin (THB). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tetrahydrobiopterin (THB) in the samples is then determined by comparing the OD of the samples to the standard curve.
Reactivity	All
Range	1.57-100 ng/mL
Sensitivity	0.51 ng/mL
Protein Length	2h
Alternative names	Tetrahydrobiopterin, BH4, Kuvan, Sapropterin
Note	For research use only
Sample Types	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration Date	Please enquire.