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> TNF RII Protein, Human, Recombinant (mFc)

TNF RII Protein, Human, Recombinant (mFc)

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Cat# NB-64-52693-1mg

Size : 1mg

Brand : Neo Biotech

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TNF RII Protein, Human, Recombinant (mFc)

(Synonyms: Tumor necrosis factor receptor type II, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNFRSF1B, TNF-RII, TNF-R2, p80 TNF-α receptor, p80 TNF-alpha receptor, p75, CD120b) Copy Product Info

Synonyms: Tumor necrosis factor receptor type II, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNFRSF1B, TNF-RII, TNF-R2, p80 TNF-α receptor, p80 TNF-alpha receptor, p75, CD120b

Catalog No. TMPJ-00288 Copy Product Info
TNF RII Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with C-mFc tag. The predicted molecular weight is 60 KDa and the accession number is P20333.
Other Forms of TNF RII Protein, Human, Recombinant (mFc):
TNF RII Protein, Human, Recombinant (hFc)
TNF RII Protein, Mouse, Recombinant (His & Avi), Biotinylated
HCLS1 Protein, Human, Recombinant (His)
TNF RII Protein, Rhesus macaque, Recombinant (hFc)
TNF RII Protein, Rhesus macaque, Recombinant (His)
Siglec-7 Protein,Human,Recombinant (aa 19-353, His)
CD122/IL2RB Protein, Human, Recombinant (His & Avi)
CD122/IL2RB Protein (Primary Amine Labeling), Cynomolgus, Recombinant (His), Biotinylated
TNFR2/CD120b/TNFR1B Protein, Cynomolgus, Recombinant (His)
TNFR2/CD120b/TNFR1B Protein, Human, Recombinant (His & hFc)
TNFR2/CD120b/TNFR1B Protein, Human, Recombinant (His)
TNFR2/CD120b/TNFR1B Protein, Mouse, Recombinant (hFc)
TNFR2/CD120b/TNFR1B Protein, Mouse, Recombinant (His)
NGFR/p75NTR Protein, Mouse, Recombinant (His)
NGFR/p75NTR Protein, Mouse, Recombinant (hFc)
TNFR2/CD120b/TNFR1B Protein, Human, Recombinant (aa 1-268, M196A, His)
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
1. Loaded Human TNF alpha-His on HIS1K Biosensor, can bind Human TNF RII-mFc with an affinity constant of 1.83 nM as determined in BLI assay. (Regularly tested)  2. Immobilized Recombinant Human TNF RII-mFc at 1μg/ml (100 μl/well) can bind Recombinant Human TNF alpha-His. The ED50 of Recombinant Human TNF alpha-His is 1.97 ng/ml. (Regularly tested)
Description
TNF RII Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with C-mFc tag. The predicted molecular weight is 60 KDa and the accession number is P20333.
Species
Human
Expression System
HEK293 Cells
TagC-mFc
Accession NumberP20333
Amino AcidPro24-Thr206
ConstructionPro24-Thr206
Protein Purity
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsTumor necrosis factor receptor type II, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNFRSF1B, TNF-RII, TNF-R2, p80 TNF-α receptor, p80 TNF-alpha receptor, p75, CD120b
Research Background
Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) is a member of the tumor necrosis factor receptor superfamily. Human TNF RII contains four cysteinerich repeats in its ECD, which shares 58% and 56% amino acid sequence identity with the mouse and rat orthologs, respectively.TNF RII is expressed predominantly on cells of the hematopoietic lineage, such as T and natural killer cells, as well as on endothelial cells, microglia, astrocytes,neurons, oligodendrocytes, cardiac myocytes, thymocytes, and mesenchymal stem cells.TNF RII binds to the membranebound forms of TNFα and Lymphotoxinα/TNFβ;soluble TNF is thought to signal predominately through TNF RI.Soluble TNF RII is believed to inhibit TNF biological activity by binding TNF thereby preventing it from activating membrane TNF receptors.
Chemical Properties
Molecular Weight60 KDa (reducing condition)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

p80 TNF-a receptor
Related Tags: TNF RII Protein, Human, Recombinant (mFc) chemical structure | TNF RII Protein, Human, Recombinant (mFc) molecular weight